利用表面等离子体波导测定生物分子相互作用动力学

2017 Photonics North (PN) Pub Date : 2017-06-01 DOI:10.1109/PN.2017.8090584

M. Khodami, P. Berini

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引用次数: 0

摘要

采用一种新型光学生物传感器技术，利用嵌入在CYTOP中的金条纹组成的表面等离子体波导，测量了BSA和Anti-BSA相互作用的动力学常数。显示这种相互作用的结合曲线是通过随时间连续记录输出功率得到的。然后将结合曲线转换成表面质量浓度。结合动力学用积分速率方程从这些曲线中提取。

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Determination of biomolecular interaction kinetics using surface plasmon waveguides

A new optical biosensor technology based on surface plasmon waveguides, composed of gold stripes embedded in CYTOP, is used to measure the kinetics constants of BSA and Anti-BSA interaction. The binding curves showing this interaction are obtained by recording the output power continuously over time. The binding curves are then converted into surface mass concentration. The binding kinetics are extracted from such curves using the integrated rate equation.

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2017 Photonics North (PN)

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