C.J.M. Arts , J.W.G.M. Wilmer , A.Th.H.J. De Bie , H. Van Den Berg
{"title":"雌性大鼠肝微粒体体外合成16α-羟孕酮及其在乳腺癌发病中的可能作用","authors":"C.J.M. Arts , J.W.G.M. Wilmer , A.Th.H.J. De Bie , H. Van Den Berg","doi":"10.1016/0022-4731(90)90169-S","DOIUrl":null,"url":null,"abstract":"<div><p>Liver homogenates from female rat strains (Sprague-Dawley, Wistar and Fisher) were incubated in a NADPH regenerating medium in the presence of labelled and unlabelled estrone. Liver microsomes isolated from male rats and female mice were used as positive controls. Using HPLC and paper chromatography, under the experimental conditions used it was found that liver homogenates from female rats were able to convert estrone to various metabolites such as 16α-hydroxyestrone. In a mutagenicity assay (Ames test), with 16α-hydroxyestrone as test substance, two strains (TA98 and TA 1538) of the five strains tested showed a 2–3-fold increase in the number of his<sup>+</sup> revenants relative to the control values. Estrone did not cause any mutagens in the test used.</p><p>It is concluded that female rats are able to synthesize 16α-hydroxyestrone <em>in vitro</em>. Whether this compound is risk factor for breast cancer remains unclear.</p></div>","PeriodicalId":17138,"journal":{"name":"Journal of steroid biochemistry","volume":"36 6","pages":"Pages 527-531"},"PeriodicalIF":0.0000,"publicationDate":"1990-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0022-4731(90)90169-S","citationCount":"4","resultStr":"{\"title\":\"In vitro synthesis of 16α-hydroxyestrone by female rat liver microsomes: Its possible role in the etiology of breast cancer\",\"authors\":\"C.J.M. Arts , J.W.G.M. Wilmer , A.Th.H.J. De Bie , H. Van Den Berg\",\"doi\":\"10.1016/0022-4731(90)90169-S\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Liver homogenates from female rat strains (Sprague-Dawley, Wistar and Fisher) were incubated in a NADPH regenerating medium in the presence of labelled and unlabelled estrone. Liver microsomes isolated from male rats and female mice were used as positive controls. Using HPLC and paper chromatography, under the experimental conditions used it was found that liver homogenates from female rats were able to convert estrone to various metabolites such as 16α-hydroxyestrone. In a mutagenicity assay (Ames test), with 16α-hydroxyestrone as test substance, two strains (TA98 and TA 1538) of the five strains tested showed a 2–3-fold increase in the number of his<sup>+</sup> revenants relative to the control values. Estrone did not cause any mutagens in the test used.</p><p>It is concluded that female rats are able to synthesize 16α-hydroxyestrone <em>in vitro</em>. Whether this compound is risk factor for breast cancer remains unclear.</p></div>\",\"PeriodicalId\":17138,\"journal\":{\"name\":\"Journal of steroid biochemistry\",\"volume\":\"36 6\",\"pages\":\"Pages 527-531\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1990-08-28\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/0022-4731(90)90169-S\",\"citationCount\":\"4\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of steroid biochemistry\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/002247319090169S\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of steroid biochemistry","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/002247319090169S","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
In vitro synthesis of 16α-hydroxyestrone by female rat liver microsomes: Its possible role in the etiology of breast cancer
Liver homogenates from female rat strains (Sprague-Dawley, Wistar and Fisher) were incubated in a NADPH regenerating medium in the presence of labelled and unlabelled estrone. Liver microsomes isolated from male rats and female mice were used as positive controls. Using HPLC and paper chromatography, under the experimental conditions used it was found that liver homogenates from female rats were able to convert estrone to various metabolites such as 16α-hydroxyestrone. In a mutagenicity assay (Ames test), with 16α-hydroxyestrone as test substance, two strains (TA98 and TA 1538) of the five strains tested showed a 2–3-fold increase in the number of his+ revenants relative to the control values. Estrone did not cause any mutagens in the test used.
It is concluded that female rats are able to synthesize 16α-hydroxyestrone in vitro. Whether this compound is risk factor for breast cancer remains unclear.
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