辣木油对鱼类病原寄生腐生菌的影响

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摘要

辣木是一种极具潜力的植物,因为它具有抗菌特性。在此背景下,目的是评价油橄榄籽油对淡水鱼中最重要的真菌病——寄生菌的作用。为此,从成熟状态的豆荚中收集种子,并通过机械压榨进行冷提取过程。采用生物分析方法在马铃薯、葡萄糖和琼脂(BDA)培养基上“离体”培养1株寄生蜂。采用低能乳化法,将油与表面活性剂和水乳化,并按处理浓度倒入培养基中。试验采用随机设计,5个处理(0、0.25、0.50、0.75、1.0 mL/L),重复5次,温度保持在24℃。每24小时观察一次,共观察96小时,测定菌丝生长情况。到48小时时,0.25mL/L浓度下,细胞生长减少,0.5 ml /L浓度下,细胞生长增强;0.75、1.0mL/L。在72 h内,对照处理与0.25mL/ l处理无差异,但与其他处理有差异。然而,96小时后全部达到完全延长。因此,油橄榄籽油在浓度大于0.25mL/L时对寄生真菌具有抑菌活性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
EFEITO DO ÓLEO DE Moringa oleífera SOBRE O PATÓGENO PISCÍCOLA Saprolegnia parasítica
Moringa oleifera is a plant with great potential for this purpose, due to its antimicrobial properties. In this context, the objective was to evaluate the action of M. oleifera seed oil on the fungus Saprolegnia parasitica, which causes the most important fungal disease in freshwater fish. For this purpose, the seeds were collected from the pods in a ripe state and submitted to a cold extraction process via mechanical press. A strain of the S. parasitica was used as test culture by the bioanalytical method "in vitro" on BDA (Potato, Dextrose and Agar) medium. The oil was emulsified by the low energy method, with surfactant and water, and poured onto the plates with medium at the concentrations of the treatments. The experiment was conducted in a randomized design with 5 treatments (0, 0.25, 0.50, 0.75, and 1.0 mL/L), all with 5 repetitions, maintained at a temperature of 24°C. The plates were observed every 24 hours for 96 hours for mycelial growth measurement. By 48 hours, a reduction in growth was observed at 0.25mL/L, intensifying at levels of 0.5; 0.75, and 1.0mL/L. In 72 hours, the control treatments and the 0.25mL/Ltreatment did not differ, but were different from the other treatments. However, after 96 hours all reached full extension. Therefore, M. oleifera seed oil shows fungistatic activity on the fungus S. parasitica at concentrations higher than 0.25mL/L.
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