番茄红素单独和联合辅酶q10在链脲佐菌素诱导的周围神经病变中的作用:生化和行为研究

Gaurav Kasar, Pooja Rasal, Manoj Mahajan, A. Upaganlawar, C. Upasani
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引用次数: 1

摘要

糖尿病性神经病变(DN)是糖尿病的主要慢性后果。DN是由慢性高血糖引起的,与氧化应激(OS)的产生和神经元功能的改变有关。本研究旨在发现天然抗氧化剂,即番茄红素和辅酶Q10 (CoQ10)作为单药或同时给药,对链脲佐菌素(STZ)诱导的DN大鼠具有理想的神经保护效果。采用STZ单次皮下剂量(55 mg/kg)诱导雄性wistar大鼠DN (200 ~ 250 g),注射链脲佐菌素4周后观察到明显的神经病变。通过行为学参数评估神经病变,包括机械性异常痛、机械性痛觉过敏、热/热痛觉过敏和步行轨迹分析。通过测定坐骨神经匀浆中丙二醛(MDA)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、还原性谷胱甘肽(GSH)和一氧化氮(NO)的含量来测定氧化应激。STZ显著改变了所有行为和生化参数。番茄红素(5mg/kg/p.o.)、辅酶q10 (10mg/kg, p.o.)及其联合治疗4周可显著降低血糖水平。番茄红素和辅酶q10的组合显著改善了行为和生化变化。与单独抗氧化剂和糖尿病神经病变组相比,这两种抗氧化剂的组合显著逆转了链脲佐菌素诱导的大鼠神经病变。由此可见,番茄红素、辅酶q10单独或联合使用的神经保护作用可能是由于它们具有较强的抗氧化性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effect of Lycopene alone and along with Coenzyme-Q10 in Streptozotocin Induced Peripheral Neuropathy: Biochemical & Behavioural Study
Diabetic Neuropathy (DN) is major chronic consequences of the diabetes. DN is developed due to chronic hyperglycemia which is related with generation of oxidative stress (OS) and alteration in the neuronal function. This study was envisaged to find the desirable neuroprotective outcomes of natural antioxidants i.e., lycopene and coenzyme Q10 (CoQ10) as monotherapy or concomitant administration in rats subjected to DN induced by streptozotocin (STZ). Single subcutaneous dose of STZ (55 mg/kg) was used to induce DN in male wistar rats (200–250 g). Significant neuropathy was observed after four weeks of streptozotocin injection. Neuropathy was evaluated by behavioral parameters using mechanical allodynia, mechanical hyperalgesia, heat/thermal hyperalgesia and walking track analysis. Oxidative stress was determined by assessment of malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), reduced glutathione (GSH), and nitric oxide (NO) in homogenate of sciatic nerve. STZ significantly alters all the behavioral as well as biochemical parameters. Treatment with lycopene (5mg/kg/p.o.), CoQ10 (10mg/kg, p.o.) and their combination for four weeks significantly reduced blood glucose level. The behavioural and biochemical alteration was significantly improved with the combination of lycopene and CoQ10. The combination of both the antioxidants significantly reversed the streptozotocin induced neuropathy in rats compared to alone antioxidants as well as diabetic neuropathy group. It is concluded that neuroprotective effect of lycopene, CoQ10 alone and in combination might be due to their strong antioxidant property.
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