多重聚合酶链反应检测肉鸡制品蜡样芽孢杆菌群毒素基因

A. Gharib, N. K. A. El-Aziz, Mai Allam
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摘要

蜡样芽孢杆菌(b.s reus s.l .)由于其水解酶的活性而成为食品变质的重要原因。本研究旨在通过分析蜡样芽孢杆菌群的肠毒素基因图谱,检测埃及Sharkia省肉鸡产品中蜡样芽孢杆菌群的发生率和污染程度。总体而言,200个检测样本中有43个(21.5%)被蜡样芽孢杆菌群污染,仅鉴定出蜡样芽孢杆菌一种。蜡样芽孢杆菌群在鸡肉中较常见(25.71%),其中鸡肉中发病率最高(30%),其次是鸡肉香肠和鸡肉午餐(各25%)。同时,从19.23%的肉制品中分离出这些细菌,其中以肉汉堡(25%)居多,其次是肉饼、沙瓦马和午餐(各占20%),肉末(17.14%)和肉肠(15%)。值得注意的是,2%的阳性样品中蜡样芽孢杆菌计数最高(>10菌落形成单位(CFU)/ g),肉肠中的比例更高(33.33%)。而15.5%的阳性样本携带蜡样芽孢杆菌,计数范围>1 × 10-10 CFU/g。采用多重聚合酶链反应(PCR)对蜡样芽孢杆菌肠毒素基因进行分子分析,结果表明,ce和nhe基因的检出率为100%,而cytk和hbl基因的检出率分别为9.52%和23.8%。这些发现涉及肉和鸡肉产品中蜡样芽孢杆菌及其毒素基因的较高发生率,在埃及是一个严重的公共卫生问题。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Multiplex Polymerase Chain Reaction for Detection of Toxin Genes of Bacillus cereus Group Isolated from Meat and Chicken Products
Bacillus cereus sensu lato (B. cereus s. l.) is a significant cause of food spoilage issue owing to the activity of certain hydrolytic enzymes. This study aimed to detect the incidence and contamination level of meat and chicken products with B. cereus group in Sharkia Governorate, Egypt, with reference to their enterotoxin genes` profiles. Overall, 43 out of 200 (21.5%) examined samples were contaminated by B. cereus group, with identification of only one species, B. cereus. B. cereus group isolates were frequent in chicken samples (25.71%), with the highest incidence in chicken meat (30%) followed by chicken sausage and chicken luncheon (25% each). Meanwhile, they were isolated from 19.23% of examined meat products, which predominated in meat burger (25%), followed by each of meat kofta, shawarma, and luncheon (20% each), minced meat (17.14%) and meat sausage (15%). Of interest, the highest B. cereus count (>10 colony forming units (CFU)/ g) was found in 2% of positive samples, with a higher percent in meat sausage (33.33%). Whereas 15.5% of positive samples harbored B. cereus with counts ranging from >1x10-10 CFU/g. Molecular analysis of B. cereus enterotoxin genes using multiplex polymerase chain reaction (PCR) revealed that both ces and nhe genes were detected in 100% of the examined isolates, while cytk and hbl genes were present only in 9.52% and 23.8% of analyzed isolates, respectively. These findings, involving a higher occurrence of B. cereus and their toxin genes in meat and chicken products represent a serious public health concern in Egypt.
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