比较ELISA法和SAT法检测沙尔基亚省肉鸡鼻-气管鸟杆菌抗体

A. Eid, Amira Morsy, Samy A. A. Adael, Abdel- Shokour Ismail
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引用次数: 1

摘要

鼻气管鸟杆菌(Ornithobacterium rhinotracheale, ORT)与家禽呼吸道疾病有关。在一项调查在患有呼吸道疾病的肉鸡中纳入ORT的试验中,对250只鸡(30群)进行了临床、PM和血清学检查。临床表现为鼻分泌物、啰音、眼肿伴腹泻。除泡沫状酸奶样渗出物外,常见的肉眼病变包括纤维性心包炎、肝炎周围炎和空气囊炎以及气管和支气管的干酪样物质。采用酶联免疫吸附试验(ELISA)检测血清样本(N= 250)的ORT抗体。采用酶联免疫吸附试验(ELISA)对80%的鸡群进行了免疫吸附试验(ELISA)和玻片凝集试验(SAT)。ELISA阳性率为93.9%,ORT-I抗原阳性率为89.8%,ORT-II抗原阳性率为69.38%。可比较的结果证明,在没有ELISA检测的情况下,SAT可以作为ORT抗体监测的有用替代方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Comparative ELISA and SAT assays in detection of Ornithobacterium rhinotracheale antibodies in broiler chickens at Sharkia Governorate
The Ornithobacterium rhinotracheale (ORT) has been involved in respiratory disease complex in poultry. In a trial to investigate the inclusion of ORT in broiler chickens suffering from respiratory troubles two hundred and fifty birds ( 30 flocks) were subjected to clinical, PM and serological examination . The clinical findings of the examined birds were nasal discharges, rales, swollen eyes accompanied by diarrhea in some cases. Fibrinous pericarditis, perihepatitis and air sacculitis as well as caseated materials in trachea and bronchi were the frequently associated gross lesions beside foamy yoghurt like exudates. Enzyme linked immunosorbant assay (ELISA) was applied on Sera samples (N= 250) to detect antibodies against ORT. ELISA results were positive among 80% of the tested flocks .A selected 49 sera samples were submitted for both ELISA and Slide agglutination test (SAT). Their outcomes revealed 93.9% positive for ELISA and 89.8% and 69.38% against ORT-I and ORT-II antigens respectively. The comparable findings proved that in case of unavailability of ELISA assay the SAT could be helpful alternative in ORT antibody monitoring.
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