测定酸核苷三磷酸酶活性的比色法。

Journal of clinical chemistry and clinical biochemistry. Zeitschrift fur klinische Chemie und klinische Biochemie Pub Date : 1990-06-01 DOI:10.1515/cclm.1990.28.6.407

M Kirchgesser, N Dahlmann

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引用次数: 11

摘要

描述了一种测定酸性核苷三磷酸酶(EC 3.6.1.-)的光度法，其中无机磷酸盐从ATP或其他核苷三磷酸中释放出来。比色法测定游离磷酸盐是基于磷钼酸盐和孔雀石绿盐酸盐的绿色络合物的形成。对最佳试验条件和样品制备进行了评价。100个正常人血清的酶活性在0.5至9.0 U/l之间，其中男性平均为4.0 U/l，女性为3.8 U/l。

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A colorimetric assay for the determination of acid nucleoside triphosphatase activity.

A photometric method for the determination of the acid nucleoside triphosphatase (EC 3.6.1.-) is described, in which inorganic phosphate is liberated from ATP or other nucleoside triphosphates. Colorimetric determination of liberated phosphate is based on the formation of a green complex of phosphomolybdate and malachite green hydrochloride. Optimal test conditions were evaluated as well as the sample preparation. The enzyme activities measured in 100 normal human sera are in the range of 0.5 to 9.0 U/l with an average of 4.0 U/l for men and 3.8 U/l for women.

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Journal of clinical chemistry and clinical biochemistry. Zeitschrift fur klinische Chemie und klinische Biochemie

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