多药耐药肠道沙门氏菌血清型肠炎(SE)的体内鉴定

Mohamed E. Basuony, Hosni A. M. Hussien, E. Bakhiet, W. Kilany, Nermeen M. Abu‐Elala, Ahmed Ali
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引用次数: 1

摘要

本研究从鸡蛋和雏鸡中分离和鉴定了沙门氏菌。此外,对分离的沙门氏菌菌株在1日龄SPF雏鸡体内的致病性进行了评估。从4个不同省份的14个肉鸡养殖场(包括1个鸭养殖场)的病雏鸡和3个种鸡场的150批鸡蛋中采集了样本。表型鉴定的沙门菌分离株分别针对沙门菌属的inv -A基因、肠炎沙门菌(SE)血清型的E -1基因和鼠伤寒沙门菌(ST)血清型的flicc基因,采用种特异性多重聚合酶链反应(pcr)技术进行表型鉴定。经证实的SE分离株进一步进行RAPD指纹图谱分析。表型分析、多重pcr和RAPD指纹图谱均证实肉鸡养殖场分离株6株(42.9%)和孵化蛋批分离株2株(1.33%)为SE,其中8株为多药耐药(MDR)菌株,MDR指数为0.214 ~ 0.786。选取多药耐药(MDR) SE分离株,在1日龄SPF雏鸡体内进行致病性评估。尽管表型多样性较小,但大多数SE菌株具有高度侵袭性,死亡率可变(50-100%)。有趣的是,最低的MDR指数与SE菌株的高毒力相关(死亡率≥85%),反之亦然。研究结果显示埃及家禽中存在SE。在家禽中不加控制地使用抗生素可能是耐多药沙门氏菌流行率上升的原因,这可能限制沙门氏菌控制措施并威胁公众健康。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
In-vivo characterization of multidrug-resistant Salmonella enterica Serovar Enteritidis (SE) recovered from fertile eggs and baby chicks
The present study was conducted to isolate and characterize Salmonella spp. from hatching eggs and baby chicks. Additionally, the pathogenicity of the isolated Salmonella strains was assessed in one-day-old specific pathogen-free (SPF) chicks in-vivo. Samples from sick baby chicks from 14 broiler chicken farms (including 1 duck farm) and 150 egg batches from three breeder chicken farms were collected from 4 different governorates. Phenotypically identified Salmonella isolates were confirmed using species-specific multiplex-PCR targeting the inv -A gene for Salmonella genus, E -1 gene for Salmonella Enteritidis (SE) serovar, and Flic-C gene for Salmonella Typhimurium (ST) serovar. Confirmed SE isolates were further subjected to Random Amplified Polymorphic DNA (RAPD) fingerprinting. Phenotypic, multiplex-PCR, and RAPD fingerprinting confirmed six isolates (42.9%) from broiler chicken farms and two isolates from hatching egg batches (1.33%) as SE, of which eight were multidrug-resistant (MDR) strains with 0.214-0.786 MDR indices. In-vivo pathogenicity of selected multidrug-resistant (MDR) SE isolates was evaluated in one-day-old SPF chicks. Despite minor phenotypic diversity, most SE strains were highly invasive with variable mortality (50-100%). Interestingly, the lowest MDR indices were associated with high virulence in SE strains (mortality ≥85%) and vice versa. The study results showed the presence of SE in poultry in Egypt. The uncontrolled usage of antibiotics in poultry could be the reason for the increased prevalence of MDR Salmonella spp., which may limit Salmonella control measures and threaten public health.
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