[l -抗坏血酸和骨代谢因子对ROS 17/2.8细胞碱性磷酸酶活性和45Ca2+掺入的影响]。

A Kodama, K Hosoi, K Kurihara, T Atsumi, K Sugita, Y Shioda, H Fukuuchi, T Ueha
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引用次数: 0

摘要

在被称为偶联因子的几种生物活性物质中,转化生长因子- β (tgf - β)、白细胞介素-1 (IL-1)和前列腺素(PG) E1和E2在3天的培养过程中不仅增加了碱性磷酸酶的活性,而且增加了45Ca2+进入ROS 17/2.8的速率:已知前两种因子在骨吸收部位形成,而PG被称为骨吸收的因素之一。甲状旁腺激素,另一种影响骨代谢的激素,可提高细胞中45Ca2+的掺入并降低碱性磷酸酶的活性。这些事实表明,成骨细胞可能参与了骨被吸收时钙离子的运输。另一方面,当这些骨肉瘤细胞在含有抗坏血酸盐和甘油磷酸酯的DMEM中培养,然后用von Kossa方法用硝酸银染色时,出现了许多组细胞,阳性染色为深棕色斑点。然后在同样的条件下,在放射性钙的存在下培养细胞,并测量放射性积累。结果表明,培养基中抗坏血酸和β -甘油磷酸酯的存在显著增加了45Ca2+的积累。从这些事实可以看出,如果在适当的条件下培养,ROS 17/2.8细胞能够结合和/或积累钙离子。这些细胞在体外可能会产生钙化基质。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
[Effects of L-ascorbic acid and bone metabolism factors on alkaline phosphatase activity of and 45Ca2+ incorporation by ROS 17/2.8 cells].

Among several bioactive substances known as coupling factors, transforming growth factor-beta (TGF-beta), interleukin-1 (IL-1), and prostaglandin (PG) E1 and E2 increased not only the activity of alkaline phosphatase but also the rate of incorporation of 45Ca2+ into ROS 17/2.8 during a 3-day culture: the former two factors are known to be formed at the site where bone is resorbed, while PG's are known as one of the factors involved in bone resorption. Parathyroid hormone, another hormone that affects bone metabolism, elevated the incorporation of 45Ca2+ by and decreased the alkaline phosphatase activity of the cells. The facts indicate the possibility that the osteoblastic cells are involved in the transport of calcium ions when bones are being resorbed. On the other hand, when these osteosarcoma cells were cultured in DMEM containing ascorbate and beta-glycerophosphate, followed by staining with silver nitrate by the procedure of von Kossa, there appeared many groups of cells that were positively stained as dark brown spots. Cells were then cultured under the same conditions in the presence of radioactive calcium, and the radioactivity accumulated was measured. The result showed that the presence of both ascorbate and beta-glycerophosphate in the culture medium dramatically increased the accumulation of 45Ca2+. It appears from these facts that ROS 17/2.8 cells are capable of incorporating and/or accumulating calcium ion if they are cultured under appropriate conditions. These cells will probably be able to produce a calcified matrix in vitro.

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