[酒精对嗜铬细胞瘤PC12h细胞神经生长因子诱导的神经突生长的影响]。

T Ohtani, K Hirayama, N Nakanishi
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引用次数: 0

摘要

研究了甲醇、乙醇、丙醇、丁醇和己醇对嗜铬细胞瘤PC12h亚克隆细胞系神经生长因子(NGF)诱导的神经突生长的影响。虽然单独添加酒精不会诱导神经突的发育,但酒精可以增强ngf诱导的神经突长度的增加和携带神经突细胞的频率。然而,酒精的这种刺激作用仅在加入NGF和酒精后很短的时间内被检测到:在治疗开始后48小时和16小时,分别用NGF单独处理的细胞和用NGF加酒精处理的细胞之间的平均神经突长度和神经突形成频率的差异不显著。醇的碳链越长,对神经突形成的促进作用越大。这些结果表明,酒精并没有增强NGF对复杂神经突作用的最终强度,而是通过可能增加PC12h细胞质膜的流动性,缩短了NGF响应过程形成所需的时间。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
[Effect of alcohols on nerve growth factor-induced neurite outgrowth from pheochromocytoma PC12h cells].

Effect of alcohols (methanol, ethanol, propanol, butanol, and hexanol) on nerve growth factor (NGF)-induced neurite outgrowth from pheochromocytoma PC12h cells, a subclonal line of PC12, was studied. Although the addition of alcohol alone did not induce neurite development, both the NGF-induced increase in neurite length and the frequency of neurite-bearing cells were enhanced by alcohols. However, this stimulating effect of alcohols was detected only within a short time after NGF and alcohol had been added: differences in the average neurite length and in the frequency of neurite formation between the cells treated with NGF alone and those treated with NGF plus alcohol were not significant at 48 hours and 16 hours, respectively, after the start of treatment. The longer the carbon chain of the alcohol, greater enhancing effect on neurite formation. These results suggest that alcohols did not strengthen the final intensity of the NGF effect to elaborate neurites but rather shortened the time required for process formation in response to NGF by presumably increasing the fluidity of plasma membranes of PC12h cells.

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