公羊冻融精浆活力及生化指标分析

Bogdan Cekić, Hristiyana Kanzova, Georgi Petrov, Nevena Maksimović, I. Ćosić, A. Milovanovic
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引用次数: 0

摘要

本研究旨在检测低温保存前后拉公羊精子血浆中LDH、ALP、GOT / AST、GPT / ALT酶活性及精子活力百分比的变化。为此,研究人员对五只公羊进行了检查,每只公羊取了两枚射精。在人工授精过程中,通过人工阴道的方法收集射精。所有的射精用6AG扩展剂稀释,然后用卡苏?S亮片法。用伊红和黑氨酸涂片测定精子活力,用分光光度法测定酶活性。结果,冷冻保存后的存活精子百分比下降了15% (P ?0.001)。冻融过程还降低了LDH、ALP、GOT / AST和GPT / ALP酶的活性。综上所述,所观察到的酶与精子活力的关系可作为优化公羊冷冻保存方案的指标。年代的精子。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Analysis of vitality and biochemical parameters in freeze-thawed seminal plasma of rams
The current study aimed to examine the percentage changes of viability sperm and the activity of the enzymes LDH, ALP, GOT / AST, GPT / ALT in the sperm plasma of Lacaune rams, before and after cryopreservation. For this purpose, five rams were examined, and two ejaculates were obtained from each ram. Ejaculates are collected by the method of artificial vagina, during the insemination campaign. All ejaculates were diluted with a 6AG extender and frozen by the Cassou?s sequin method. Sperm viability was determined by eosin and nigrosine smears, and enzyme activity was examined spectrophotometrically. As a result, the percentage of vital sperm after cryopreservation decreased by 15% (P ? 0.001). The freezing and thawing process also reduced the activity of the enzymes LDH, ALP, GOT / AST and GPT / ALP. In conclusion, the observed enzymes, in relation to sperm vitality, could be used as indicators to optimize the protocols for cryopreservation of ram?s sperm.
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