Bogdan Cekić, Hristiyana Kanzova, Georgi Petrov, Nevena Maksimović, I. Ćosić, A. Milovanovic
{"title":"公羊冻融精浆活力及生化指标分析","authors":"Bogdan Cekić, Hristiyana Kanzova, Georgi Petrov, Nevena Maksimović, I. Ćosić, A. Milovanovic","doi":"10.2298/bah2201031c","DOIUrl":null,"url":null,"abstract":"The current study aimed to examine the percentage changes of viability sperm\n and the activity of the enzymes LDH, ALP, GOT / AST, GPT / ALT in the sperm\n plasma of Lacaune rams, before and after cryopreservation. For this purpose,\n five rams were examined, and two ejaculates were obtained from each ram.\n Ejaculates are collected by the method of artificial vagina, during the\n insemination campaign. All ejaculates were diluted with a 6AG extender and\n frozen by the Cassou?s sequin method. Sperm viability was determined by\n eosin and nigrosine smears, and enzyme activity was examined\n spectrophotometrically. As a result, the percentage of vital sperm after\n cryopreservation decreased by 15% (P ? 0.001). The freezing and thawing\n process also reduced the activity of the enzymes LDH, ALP, GOT / AST and GPT\n / ALP. In conclusion, the observed enzymes, in relation to sperm vitality,\n could be used as indicators to optimize the protocols for cryopreservation\n of ram?s sperm.","PeriodicalId":249404,"journal":{"name":"Biotehnologija u stocarstvu","volume":"41 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Analysis of vitality and biochemical parameters in freeze-thawed seminal plasma of rams\",\"authors\":\"Bogdan Cekić, Hristiyana Kanzova, Georgi Petrov, Nevena Maksimović, I. Ćosić, A. Milovanovic\",\"doi\":\"10.2298/bah2201031c\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The current study aimed to examine the percentage changes of viability sperm\\n and the activity of the enzymes LDH, ALP, GOT / AST, GPT / ALT in the sperm\\n plasma of Lacaune rams, before and after cryopreservation. For this purpose,\\n five rams were examined, and two ejaculates were obtained from each ram.\\n Ejaculates are collected by the method of artificial vagina, during the\\n insemination campaign. All ejaculates were diluted with a 6AG extender and\\n frozen by the Cassou?s sequin method. Sperm viability was determined by\\n eosin and nigrosine smears, and enzyme activity was examined\\n spectrophotometrically. As a result, the percentage of vital sperm after\\n cryopreservation decreased by 15% (P ? 0.001). The freezing and thawing\\n process also reduced the activity of the enzymes LDH, ALP, GOT / AST and GPT\\n / ALP. In conclusion, the observed enzymes, in relation to sperm vitality,\\n could be used as indicators to optimize the protocols for cryopreservation\\n of ram?s sperm.\",\"PeriodicalId\":249404,\"journal\":{\"name\":\"Biotehnologija u stocarstvu\",\"volume\":\"41 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1900-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biotehnologija u stocarstvu\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.2298/bah2201031c\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biotehnologija u stocarstvu","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.2298/bah2201031c","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Analysis of vitality and biochemical parameters in freeze-thawed seminal plasma of rams
The current study aimed to examine the percentage changes of viability sperm
and the activity of the enzymes LDH, ALP, GOT / AST, GPT / ALT in the sperm
plasma of Lacaune rams, before and after cryopreservation. For this purpose,
five rams were examined, and two ejaculates were obtained from each ram.
Ejaculates are collected by the method of artificial vagina, during the
insemination campaign. All ejaculates were diluted with a 6AG extender and
frozen by the Cassou?s sequin method. Sperm viability was determined by
eosin and nigrosine smears, and enzyme activity was examined
spectrophotometrically. As a result, the percentage of vital sperm after
cryopreservation decreased by 15% (P ? 0.001). The freezing and thawing
process also reduced the activity of the enzymes LDH, ALP, GOT / AST and GPT
/ ALP. In conclusion, the observed enzymes, in relation to sperm vitality,
could be used as indicators to optimize the protocols for cryopreservation
of ram?s sperm.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
