姜黄素氧化镁纳米粒子偶联物对2型糖尿病大鼠的抗糖尿病作用

Y. Shehata, M. Mansour, S. Shadad, M. Metwally, A. Arisha
{"title":"姜黄素氧化镁纳米粒子偶联物对2型糖尿病大鼠的抗糖尿病作用","authors":"Y. Shehata, M. Mansour, S. Shadad, M. Metwally, A. Arisha","doi":"10.21608/zvjz.2020.23007.1098","DOIUrl":null,"url":null,"abstract":"Diabetes is a disorder that impairs the ability of the body to process blood glucose. Diabetes is either due to the pancreas produces insufficient amount of insulin or the cells of the body do not react with the insulin produced. Type 2 diabetes (diabetes mellitus) is the most common type of diabetes and grows in prevalence worldwide. The aim of our study was to investigate the anti-diabetic impact with emphasis on hepatic lipid metabolism and spleen histopathology effect of curcumin-magnesium oxide nanoparticles conjugate (Cur-MgO NPs conjugate) in nicotinamide (NA)-streptozotocin (STZ) induced type 2 diabetic rats. This study included three groups: Group 1 (control group), Group 2 (STZ induced type 2 diabetic rats) and Group 3 (type 2 diabetic rats were orally administered Cur-MgO NPs conjugate (10 mg/Kg BW/day for 45 days)). The levels of plasma glucose, serum insulin, lipid profile and hepatic expression of lipogenic enzymes Malonyl-CoA decarboxylase (MCD), Peroxisome proliferator-activated receptor alpha (Ppar α) were measured. Moreover, histopathological examination of spleen was carried out. The assessment of Insulin Resistance (HOMA-IR), a significant reduction in serum insulin level and HOMA of β-cell function (HOMA β). Also, reduction in hepatic mRNA expression of MCD and Ppar-α and an altered lipid profile were noticed in diabetic group in comparison with the control group. Oral administration of Cur-MgO NPs conjugate restored glucose (102.73±2.16), TC (139.80±3.61) and LDL-c (87.56±3.72) to their physiological levels in the control group (108.29±2.45, 150.27±2.56 and 94.43±2.75 respectively). Histopathological examination of the spleen showed damage of lymphoid cells in type 2 diabetes (T2D), while in treated group showed moderate improvement in spleen tissue and cells are rescued from apoptosis. The outcome of present study revealed that Cur-MgO NP conjugate has potential ameliorative effect on the hematologic, immunologic and hepatic metabolic alterations in type 2 diabetic rats.","PeriodicalId":137314,"journal":{"name":"Zagazig Veterinary Journal","volume":"46 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2020-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Anti-Diabetic Effects of Curcumin-Magnesium Oxide Nanoparticles Conjugate in Type 2 Diabetic Rats\",\"authors\":\"Y. Shehata, M. Mansour, S. Shadad, M. Metwally, A. Arisha\",\"doi\":\"10.21608/zvjz.2020.23007.1098\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Diabetes is a disorder that impairs the ability of the body to process blood glucose. Diabetes is either due to the pancreas produces insufficient amount of insulin or the cells of the body do not react with the insulin produced. Type 2 diabetes (diabetes mellitus) is the most common type of diabetes and grows in prevalence worldwide. The aim of our study was to investigate the anti-diabetic impact with emphasis on hepatic lipid metabolism and spleen histopathology effect of curcumin-magnesium oxide nanoparticles conjugate (Cur-MgO NPs conjugate) in nicotinamide (NA)-streptozotocin (STZ) induced type 2 diabetic rats. This study included three groups: Group 1 (control group), Group 2 (STZ induced type 2 diabetic rats) and Group 3 (type 2 diabetic rats were orally administered Cur-MgO NPs conjugate (10 mg/Kg BW/day for 45 days)). The levels of plasma glucose, serum insulin, lipid profile and hepatic expression of lipogenic enzymes Malonyl-CoA decarboxylase (MCD), Peroxisome proliferator-activated receptor alpha (Ppar α) were measured. Moreover, histopathological examination of spleen was carried out. The assessment of Insulin Resistance (HOMA-IR), a significant reduction in serum insulin level and HOMA of β-cell function (HOMA β). Also, reduction in hepatic mRNA expression of MCD and Ppar-α and an altered lipid profile were noticed in diabetic group in comparison with the control group. Oral administration of Cur-MgO NPs conjugate restored glucose (102.73±2.16), TC (139.80±3.61) and LDL-c (87.56±3.72) to their physiological levels in the control group (108.29±2.45, 150.27±2.56 and 94.43±2.75 respectively). Histopathological examination of the spleen showed damage of lymphoid cells in type 2 diabetes (T2D), while in treated group showed moderate improvement in spleen tissue and cells are rescued from apoptosis. The outcome of present study revealed that Cur-MgO NP conjugate has potential ameliorative effect on the hematologic, immunologic and hepatic metabolic alterations in type 2 diabetic rats.\",\"PeriodicalId\":137314,\"journal\":{\"name\":\"Zagazig Veterinary Journal\",\"volume\":\"46 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2020-09-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Zagazig Veterinary Journal\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.21608/zvjz.2020.23007.1098\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Zagazig Veterinary Journal","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.21608/zvjz.2020.23007.1098","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

摘要

糖尿病是一种损害身体处理血糖能力的疾病。糖尿病要么是由于胰腺产生的胰岛素量不足,要么是身体细胞不与产生的胰岛素发生反应。2型糖尿病(糖尿病)是最常见的糖尿病类型,在世界范围内的患病率不断上升。本研究旨在探讨姜黄素-氧化镁纳米缀合物(curc - mgo NPs缀合物)对烟酰胺-链脲佐菌素(STZ)诱导的2型糖尿病大鼠的抗糖尿病作用,重点探讨其对肝脏脂质代谢和脾脏组织病理学的影响。实验分为三组:1组(对照组)、2组(STZ诱导的2型糖尿病大鼠)和3组(2型糖尿病大鼠口服Cur-MgO NPs偶联物(10 mg/Kg BW/d,连续45 d)。测定大鼠血浆葡萄糖、血清胰岛素、血脂水平和肝脏脂肪生成酶丙二酰辅酶a脱羧酶(MCD)、过氧化物酶体增殖物激活受体α (Ppar α)的表达。同时进行脾脏组织病理学检查。评估胰岛素抵抗(HOMA- ir),血清胰岛素水平显著降低和β细胞功能HOMA (HOMA β)。此外,与对照组相比,糖尿病组肝脏MCD和Ppar-α mRNA表达降低,脂质谱改变。口服cu - mgo NPs偶联物使对照组血糖(102.73±2.16)、TC(139.80±3.61)和LDL-c(87.56±3.72)恢复到生理水平(分别为108.29±2.45、150.27±2.56和94.43±2.75)。2型糖尿病(T2D)脾组织病理检查显示淋巴样细胞损伤,治疗组脾组织有中度改善,细胞免于凋亡。本研究结果表明,curl - mgo NP偶联物对2型糖尿病大鼠的血液学、免疫学和肝脏代谢改变具有潜在的改善作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Anti-Diabetic Effects of Curcumin-Magnesium Oxide Nanoparticles Conjugate in Type 2 Diabetic Rats
Diabetes is a disorder that impairs the ability of the body to process blood glucose. Diabetes is either due to the pancreas produces insufficient amount of insulin or the cells of the body do not react with the insulin produced. Type 2 diabetes (diabetes mellitus) is the most common type of diabetes and grows in prevalence worldwide. The aim of our study was to investigate the anti-diabetic impact with emphasis on hepatic lipid metabolism and spleen histopathology effect of curcumin-magnesium oxide nanoparticles conjugate (Cur-MgO NPs conjugate) in nicotinamide (NA)-streptozotocin (STZ) induced type 2 diabetic rats. This study included three groups: Group 1 (control group), Group 2 (STZ induced type 2 diabetic rats) and Group 3 (type 2 diabetic rats were orally administered Cur-MgO NPs conjugate (10 mg/Kg BW/day for 45 days)). The levels of plasma glucose, serum insulin, lipid profile and hepatic expression of lipogenic enzymes Malonyl-CoA decarboxylase (MCD), Peroxisome proliferator-activated receptor alpha (Ppar α) were measured. Moreover, histopathological examination of spleen was carried out. The assessment of Insulin Resistance (HOMA-IR), a significant reduction in serum insulin level and HOMA of β-cell function (HOMA β). Also, reduction in hepatic mRNA expression of MCD and Ppar-α and an altered lipid profile were noticed in diabetic group in comparison with the control group. Oral administration of Cur-MgO NPs conjugate restored glucose (102.73±2.16), TC (139.80±3.61) and LDL-c (87.56±3.72) to their physiological levels in the control group (108.29±2.45, 150.27±2.56 and 94.43±2.75 respectively). Histopathological examination of the spleen showed damage of lymphoid cells in type 2 diabetes (T2D), while in treated group showed moderate improvement in spleen tissue and cells are rescued from apoptosis. The outcome of present study revealed that Cur-MgO NP conjugate has potential ameliorative effect on the hematologic, immunologic and hepatic metabolic alterations in type 2 diabetic rats.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信