[曼氏血吸虫活卵的制备图]。

Angewandte Parasitologie Pub Date : 1990-11-01
W Schlisio, M Lafrenz
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引用次数: 0

摘要

为了维持miracidia的孵化能力,从实验感染小鼠的肝脏和脾脏中大量采集了mansoni s.m ansoni纯虫卵。为此,用木瓜蛋白酶、透明质酸酶和胰蛋白酶消化组织。利用筛室(筛孔分别为200微米× 200微米和28微米× 28微米)和密度梯度离心,将残渣中的虫卵与剩余的宿主细胞、组织材料和死卵分离。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
[Preparative representation of the living eggs of Schistosoma mansoni].

To maintain the hatchability of miracidia, pure worm eggs of S. mansoni were collected in great quantities from liver and spleen of experimentally infected mice. For this purpose, the tissue was digested with papain, hyaluronidase and trypsin. Worm eggs in the residue were separated from remaining host cells, tissue materials and dead eggs by using the sieving chamber (sieves 200 microns x 200 microns and 28 microns x 28 microns) and by density gradient centrifugation.

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