{"title":"蜂王浆及其SDS-PAGE电泳图谱","authors":"H. E. Çakir, Y. Şirin, S. Kolaylı","doi":"10.35206/jan.638063","DOIUrl":null,"url":null,"abstract":"Royal jelly (RJ) is an important functional food, is used for many purposes. New methods are needed for the practical characterization of royal jelly. In this preliminary study, SDS-PAGE electrophoretic profiles of royal jelly proteins were investigated. Raw royal jelly samples were analyzed by classical SDS-PAGE electrophoresis using 5% and 10% gels. The major protein spot were obtained at the samples collected same regions. Compared the proteins with known marker proteins they were between 50 and 80 kDa. Classical SDS-PAGE electrophoresis alone is not sufficient to determine royal jelly proteins, but can be used to basic characterization of royal jelly. Furthers electrophoretic techniques are needed for a better separation.","PeriodicalId":205620,"journal":{"name":"Journal of Apitherapy and Nature","volume":"217 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2019-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Royal Jelly and Its SDS-PAGE Electrophoresis Profiles\",\"authors\":\"H. E. Çakir, Y. Şirin, S. Kolaylı\",\"doi\":\"10.35206/jan.638063\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Royal jelly (RJ) is an important functional food, is used for many purposes. New methods are needed for the practical characterization of royal jelly. In this preliminary study, SDS-PAGE electrophoretic profiles of royal jelly proteins were investigated. Raw royal jelly samples were analyzed by classical SDS-PAGE electrophoresis using 5% and 10% gels. The major protein spot were obtained at the samples collected same regions. Compared the proteins with known marker proteins they were between 50 and 80 kDa. Classical SDS-PAGE electrophoresis alone is not sufficient to determine royal jelly proteins, but can be used to basic characterization of royal jelly. Furthers electrophoretic techniques are needed for a better separation.\",\"PeriodicalId\":205620,\"journal\":{\"name\":\"Journal of Apitherapy and Nature\",\"volume\":\"217 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2019-11-11\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Apitherapy and Nature\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.35206/jan.638063\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Apitherapy and Nature","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.35206/jan.638063","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Royal Jelly and Its SDS-PAGE Electrophoresis Profiles
Royal jelly (RJ) is an important functional food, is used for many purposes. New methods are needed for the practical characterization of royal jelly. In this preliminary study, SDS-PAGE electrophoretic profiles of royal jelly proteins were investigated. Raw royal jelly samples were analyzed by classical SDS-PAGE electrophoresis using 5% and 10% gels. The major protein spot were obtained at the samples collected same regions. Compared the proteins with known marker proteins they were between 50 and 80 kDa. Classical SDS-PAGE electrophoresis alone is not sufficient to determine royal jelly proteins, but can be used to basic characterization of royal jelly. Furthers electrophoretic techniques are needed for a better separation.
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