{"title":"未能证明各种pre-pro-TRH片段对体外大鼠垂体TSH和PRL释放的影响。","authors":"T Mitsuma, Y Hirooka, M Kimura, T Nogimori","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>The effects of Pro-thyrotropin-releasing hormone (TRH), pre-pro-TRH (53-74), pre-pro-TRH (83-106), pre-pro-TRH (115-151), pre-pro-TRH (160-169), pre-pro-TRH (178-199) and TRH on thyrotropin (TSH) and prolactin (PRL) release from the rat anterior pituitary were studied in vitro. The rat anterior pituitary was incubated in medium 199 (pH 7.4) with 1.0 mg/ml of bacitracin (medium) for 30 min. The amount of TSH and PRL release into the medium was measured by radioimmunassay. TSH and PRL release from the anterior pituitary was stimulated with the addition of TRH or pro-TRH in a dose-related manner, but not with other peptides. The findings suggest that TRH and pro-TRH stimulate TSH and PRL release from the anterior pituitary in vitro.</p>","PeriodicalId":11547,"journal":{"name":"Endocrinologia experimentalis","volume":"24 3","pages":"333-9"},"PeriodicalIF":0.0000,"publicationDate":"1990-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Failure to demonstrate the effect of various pre-pro-TRH fragments on TSH and PRL release from rat pituitary in vitro.\",\"authors\":\"T Mitsuma, Y Hirooka, M Kimura, T Nogimori\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>The effects of Pro-thyrotropin-releasing hormone (TRH), pre-pro-TRH (53-74), pre-pro-TRH (83-106), pre-pro-TRH (115-151), pre-pro-TRH (160-169), pre-pro-TRH (178-199) and TRH on thyrotropin (TSH) and prolactin (PRL) release from the rat anterior pituitary were studied in vitro. The rat anterior pituitary was incubated in medium 199 (pH 7.4) with 1.0 mg/ml of bacitracin (medium) for 30 min. The amount of TSH and PRL release into the medium was measured by radioimmunassay. TSH and PRL release from the anterior pituitary was stimulated with the addition of TRH or pro-TRH in a dose-related manner, but not with other peptides. The findings suggest that TRH and pro-TRH stimulate TSH and PRL release from the anterior pituitary in vitro.</p>\",\"PeriodicalId\":11547,\"journal\":{\"name\":\"Endocrinologia experimentalis\",\"volume\":\"24 3\",\"pages\":\"333-9\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1990-09-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Endocrinologia experimentalis\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Endocrinologia experimentalis","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Failure to demonstrate the effect of various pre-pro-TRH fragments on TSH and PRL release from rat pituitary in vitro.
The effects of Pro-thyrotropin-releasing hormone (TRH), pre-pro-TRH (53-74), pre-pro-TRH (83-106), pre-pro-TRH (115-151), pre-pro-TRH (160-169), pre-pro-TRH (178-199) and TRH on thyrotropin (TSH) and prolactin (PRL) release from the rat anterior pituitary were studied in vitro. The rat anterior pituitary was incubated in medium 199 (pH 7.4) with 1.0 mg/ml of bacitracin (medium) for 30 min. The amount of TSH and PRL release into the medium was measured by radioimmunassay. TSH and PRL release from the anterior pituitary was stimulated with the addition of TRH or pro-TRH in a dose-related manner, but not with other peptides. The findings suggest that TRH and pro-TRH stimulate TSH and PRL release from the anterior pituitary in vitro.
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