埃及西北海岸骆驼的免疫学和细菌学研究

A. Hafez, M. El-Rayes
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摘要

本研究旨在揭示引起骆驼呼吸症状的最重要细菌及其对炎症细胞因子的影响,以及它们与其他免疫参数(如急性期蛋白、补体和免疫球蛋白)的关系。本研究收集肺标本(n = 572),其中390例(68.18%)为正常肺作为对照组,肺炎骆驼肺标本(n = 182;31.82%)被归类为患病群体,全部来自埃及西北海岸的屠宰场。分离菌株包括9属致病菌;葡萄球菌88株(19%)、沙门氏菌40株(8.7%)、肺炎克雷伯菌75株(16.4%)、多杀性巴斯德菌38株(8.3%)、支原体58株(12.6%)、芽孢杆菌35株(7.6%)、化脓性链球菌20株(4.3%)、肺炎链球菌68株(14.8%)、变形杆菌35株(7.6%)。利用采集的两组血液样本,评估特定的生化和免疫学参数。患病骆驼的促炎细胞因子(IL-2、IL-6、TNF和IFN-)、急性期蛋白(纤维蛋白原、铜蓝蛋白、触珠蛋白和血清淀粉样蛋白)和急性期细胞因子(IL-2、IL-6、TNF和IFN)显著增加。免疫球蛋白升高,但总蛋白(TP)、白蛋白(Alb)、丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)和谷氨酰转移酶(GGT)受感染影响不显著。与对照组相比,抗炎细胞因子IL-10、补体3、补体4、白蛋白水平和A/G比值均显著降低,血液学分析显示RBC计数极显著降低(P≤0.01)。组织病理学发现细支气管壁增生。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
SOME IMMUNOLOGICAL AND BACTERIOLOGICAL STUDIES ON CAMELS IN THE NORTH WESTERN COAST OF EGYPT
This study aimed to shed light on the most important bacteria that cause respiratory manifestations and their effect on inflammatory cytokines in camels, and their relationship with other immunological parameters such as Acute Phase Proteins, complement, and immunoglobulins. Lung specimens (n = 572) were collected for this investigation, with normal lungs identified in 390 (68.18%) serving as a control group, and pneumonic camel lung samples (n = 182; 31.82%) classified as the diseased group, all of which were collected from slaughterhouses in Egypt's North Western Coast. The isolates comprised nine genera of pathogenic bacteria; Staphylococcus spp. 88 (19%), Salmonella spp. 40 (8.7%), Klebsiella pneumoniae 75 (16.4%), Pastuerella multocida 38 (8.3%), Mycoplasma spp. 58 (12.6%), Bacillus spp. 35 (7.6%), Streptococcus pyogenes 20 (4.3%), Streptococcus pneumoniae 68 (14.8%) and Proteus spp. 35 (7.6%). Using blood samples collected from both groups, the specified biochemical and immunological parameters were evaluated. The diseased camels exhibited substantial increases in pro-inflammatory cytokines (IL-2, IL-6, TNF, and IFN-), acute phase proteins (fibrinogen, ceruloplasmin, haptoglobin, and serum amyloid), and acute phase cytokines (IL-2, IL-6, TNF and IFN). Immunoglobulins increase, but total protein (TP), albumin (Alb), alanine aminotransferase (ALT), aspartate aminotransferase (AST), and gamma glutamyl transferase (GGT) were not significantly affected by infection. In contrast, the anti-inflammatory cytokine IL-10, complement 3, complement 4, albumin serum levels, and the A/G ratio all decreased substantially, and hematological analysis revealed a highly significant decrease (P ≤ 0.01) in RBC count compared to the control group. Histopathological finding revealed a hyperplastic bronchiolar wall.
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