唾液预处理对类固醇激素浓度的影响。

Journal of clinical chemistry and clinical biochemistry. Zeitschrift fur klinische Chemie und klinische Biochemie Pub Date : 1990-12-01 DOI:10.1515/cclm.1990.28.12.923

E P Meulenberg, J A Hofman

{"title":"唾液预处理对类固醇激素浓度的影响。","authors":"E P Meulenberg, J A Hofman","doi":"10.1515/cclm.1990.28.12.923","DOIUrl":null,"url":null,"abstract":"We investigated the effect of the pretreatment (sonification or centrifugation) of saliva samples on the concentration of several steroid hormones as measured with highly specific RIA after extraction and chromatography. It appeared that sonification of saliva resulted in significantly higher values for progesterone, cortisone, 17-hydroxyprogesterone, testosterone and oestradiol (10-49% increase), compared with the levels recorded after centrifugation. No differences were demonstrated for the concentrations of cortisol and androstenedione, except that a sex-dependent difference effect was observed in the values for androstenedione: concentrations measured in sonificated male saliva were lower than those measured in supernatant saliva.","PeriodicalId":15649,"journal":{"name":"Journal of clinical chemistry and clinical biochemistry. Zeitschrift fur klinische Chemie und klinische Biochemie","volume":"28 12","pages":"923-8"},"PeriodicalIF":0.0000,"publicationDate":"1990-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/cclm.1990.28.12.923","citationCount":"19","resultStr":"{\"title\":\"The effect of pretreatment of saliva on steroid hormone concentrations.\",\"authors\":\"E P Meulenberg, J A Hofman\",\"doi\":\"10.1515/cclm.1990.28.12.923\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"We investigated the effect of the pretreatment (sonification or centrifugation) of saliva samples on the concentration of several steroid hormones as measured with highly specific RIA after extraction and chromatography. It appeared that sonification of saliva resulted in significantly higher values for progesterone, cortisone, 17-hydroxyprogesterone, testosterone and oestradiol (10-49% increase), compared with the levels recorded after centrifugation. No differences were demonstrated for the concentrations of cortisol and androstenedione, except that a sex-dependent difference effect was observed in the values for androstenedione: concentrations measured in sonificated male saliva were lower than those measured in supernatant saliva.\",\"PeriodicalId\":15649,\"journal\":{\"name\":\"Journal of clinical chemistry and clinical biochemistry. Zeitschrift fur klinische Chemie und klinische Biochemie\",\"volume\":\"28 12\",\"pages\":\"923-8\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1990-12-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1515/cclm.1990.28.12.923\",\"citationCount\":\"19\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of clinical chemistry and clinical biochemistry. Zeitschrift fur klinische Chemie und klinische Biochemie\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1515/cclm.1990.28.12.923\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of clinical chemistry and clinical biochemistry. Zeitschrift fur klinische Chemie und klinische Biochemie","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1515/cclm.1990.28.12.923","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}

引用次数: 19

摘要

我们研究了唾液样品的预处理(超声或离心)对几种类固醇激素浓度的影响，这些激素在提取和层析后用高特异性RIA测定。与离心后记录的水平相比，唾液超声处理导致孕酮、可的松、17-羟孕酮、睾酮和雌二醇的值明显升高(增加10-49%)。皮质醇和雄烯二酮的浓度没有差异，除了雄烯二酮的值存在性别依赖性差异效应:在超声处理的男性唾液中测量的浓度低于在上清唾液中测量的浓度。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

The effect of pretreatment of saliva on steroid hormone concentrations.

We investigated the effect of the pretreatment (sonification or centrifugation) of saliva samples on the concentration of several steroid hormones as measured with highly specific RIA after extraction and chromatography. It appeared that sonification of saliva resulted in significantly higher values for progesterone, cortisone, 17-hydroxyprogesterone, testosterone and oestradiol (10-49% increase), compared with the levels recorded after centrifugation. No differences were demonstrated for the concentrations of cortisol and androstenedione, except that a sex-dependent difference effect was observed in the values for androstenedione: concentrations measured in sonificated male saliva were lower than those measured in supernatant saliva.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Journal of clinical chemistry and clinical biochemistry. Zeitschrift fur klinische Chemie und klinische Biochemie

自引率

0.00%

发文量