二氧化钛纳米颗粒和姜黄素对温度应激大鼠阴囊高温精子参数的影响:miR455的作用

M. Mollaei, M. Hashemi, E. Siasi, Sayeh Jafari Marndi, M. Entezari
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引用次数: 1

摘要

背景:温度变化引起睾丸功能障碍。据观察,睾丸热疗导致氧化应激,并导致睾丸参数严重减少。由于压力导致精子参数严重减少,变得氧化。近年来,人们开始考虑天然植物材料和磁性纳米颗粒。在线粒体内部凋亡途径中,gen bcl2是miR455的靶标。目的:本研究旨在通过研究温度诱导应激大鼠阴囊高温后miR455的表达变化,探讨二氧化钛纳米颗粒对精子参数的影响,并通过抗氧化剂姜黄素改善其对精子参数的影响。方法:制备完成后,将大鼠置于43℃热水浴中。连续6天,每次30分钟。然后将老鼠分成八组。我们使用浓度为0.03 mg/kg体重的TiO2纳米粒子和浓度为0.02 mg/kg体重的姜黄素。杀死动物后,对精子的活力、浓度、活力和形态等参数进行了研究。采用合适的试剂盒进行RNA提取和cDNA合成。设计基因引物,RT-PCR检测基因表达。采用t检验和方差分析检验各组间差异。采用Prism8软件和SPSS 26进行数据分析。结果:miR455在治疗组表达较低,且与姜黄素相关(P < 0.05)。姜黄素对改善阴囊高温大鼠精子参数有积极作用,而TiO2纳米颗粒有消极和毒性作用。然而,当给大鼠服用TiO2纳米颗粒和姜黄素时,精子参数有显著改善。结论:姜黄素中miR455表达的变化控制了TiO2纳米颗粒的损伤。miRNA455似乎可以作为预测精子健康状况的标记物。因此,姜黄素可以起到保护作用,减少睾丸热疗和二氧化钛纳米颗粒的毒性作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effect oF TiO2 Nanoparticles and Curcumin on Sperm Parameters in Response to Temperature-Induced Stress in Scrotal Hyperthermia Rats: Role of miR455
Background: Temperature changes cause testicular dysfunction. It has been observed that testicular hyperthermia leads to oxidative stress and as a result a severe reduction in testicular parameters. Causes a severe reduction in Sperm parameters to become oxidative due to stress. Recently, natural plant materials and magnetic nanoparticles have been considered. In the internal mitochondrial apoptosis pathway, gen bcl2 is a target of miR455. Objectives: The present study aimed to investigate the effects of titanium dioxide nanoparticles and improve their impacts by using the antioxidant curcumin on sperm parameters by investigating changes in expression miR455 in response to temperature-induced stress in scrotal hyperthermia rats. Methods: After preparation, the rats were placed in a hot water bath at 43°C. for 30 minutes for six consecutive days. The rats were then divided into eight groups. We used TiO2 nanoparticles at a concentration of 0.03 mg/kg of body weight and curcumin at a concentration of 0.02 mg/kg of body weight. After killing the animals, such parameters of sperm as viability, concentration, motility, and morphology of spermatozoa were studied. RNA extraction and cDNA synthesis were performed using appropriate kits. A gene primer was designed and RT-PCR was used to assess gene expression. The t-test and ANOVA were used to examine differences between different groups. Data analysis was performed using Prism8 software and SPSS version 26. Results: The results showed that miR455 expression was lower in the treatment groups and was associated with curcumin (P < 0.05). A positive effect of curcumin on improving sperm parameters in rats with scrotum hyperthermia and a negative and toxic effect of TiO2 nanoparticles were shown. However, a significant improvement in sperm parameters was observed when rats were given TiO2 nanoparticles along with curcumin. Conclusions: The changes in the expression miR455, shown in curcumin have controlled the damage to TiO2 nanoparticles. It seems that miRNA455 can be used as a marker to predict sperm health status. So Curcumin can play a protective role in reducing the toxic effects of testicular hyperthermia as well as titanium dioxide nanoparticles.
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