不同药用植物叶片提取物抗菌活性评价及初步植物化学分析

Shilpendra Kour, Rekha Khandal, R. Tripathi
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摘要

摘要本研究通过制备曼陀罗、索尼弗兰和玫瑰花的水提物和有机提物,考察其对枯草芽孢杆菌、蜡样芽孢杆菌、巨芽孢杆菌、大肠杆菌、金黄色葡萄球菌、荧光假单胞菌等致病菌的抑菌作用。扩散试验的结果表明抑制的模式,主要取决于用于提取的溶剂和生物体。有机提取物比水提取物具有更强的抑菌活性。其中,甲醇和乙酸乙酯提取物对所有细菌的抑菌活性最强。初步的植物化学分析显示,提取物中存在苷类、生物碱、植物甾醇、固定油、酚类化合物和碳水化合物。进一步分析表明,活性化合物本质上不是蛋白质。对这些植物的粗提物进行抑菌活性研究,以验证其在中药中的应用价值。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
EVALUATION OF LEAF EXTRACTS OF DIFFERENT MEDICINAL PLANTS FOR POTENTIAL ANTIBACTERIAL ACTIVITY AND PRELIMINARY PHYTOCHEMICAL ANALYSIS
The aim of the study was to evaluate the antibacterial properties of Datura stromanium,Withania somniferum, and Catharanthus roseus by preparing their crude aqueous and organic extractagainst bacterial pathogensBacillus subtilis, Bacillus cereus,Bacillus megaterium,Eshcherichia coli,Staphylococcus aureus, Pseudomonas fluorescence, causing diseases in human beings.The result of discdiffusion assay indicates the pattern of inhibition, depending largely upon solvent used for extractionand the organism.Organic extract provided potent antibacterial activity as compared to aqueousextract. Among all the extract methanol and ethyl acetate extract was found most active against allbacterial species. Preliminary phytochemical analysis revealed the presence of glycosides, alkaloids,phytosterols, fixed oils, phenolic compounds, carbohydrates in extracts. Further analysis shows thatthe active compound was not protein in nature. Antibacterial activity of crude extract of these plantswas carried out to validate the use of traditional medicinal herbs.
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