腺病毒(adenovirus)DNA的转录活性与宿主细胞核骨架(nuclear matrix)的关系

Science in China Series B-Chemistry, Biological, Agricultural, Medical & Earth Sciences Pub Date : 1988-10-10 DOI:10.1360/ZB1988-18-10-1052

陈枫J. A. NickersonS. Penman 翟中和

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摘要

本文用温和的选择性细胞抽提技术,提取腺病毒(adenovirus)感染6h的Hela细胞核内各种DNA组分,分别以腺病毒DNA的早期转录区(El a ,El b )与晚期转录区( L 2 )作探针进行Southern杂交,结果表明只有正在活跃转录着的腺病毒DNA片段才与宿主细胞核骨架紧密结合,从而说明宿主细胞核骨架可能对病毒DNA的转录有重要作用。

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腺病毒(adenovirus)DNA的转录活性与宿主细胞核骨架(nuclear matrix)的关系

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Science in China Series B-Chemistry, Biological, Agricultural, Medical & Earth Sciences

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