{"title":"多孔硅基生物传感器的制备","authors":"F. Mathew, E. Alocilja","doi":"10.1109/ICSENS.2003.1278945","DOIUrl":null,"url":null,"abstract":"A porous silicon-based biosensor for detection of bacteria was fabricated. Silicon (0.01 ohm-cm, p-type) was anodized electrochemically in a hydrofluoric acid solution containing electrochemical cell using different current and time conditions ranging from 100 mA/cm2 for 30 sec to 2 mA/cm for 1 hr to produce sponge like porous layer of silicon. Anodizing conditions of 5 mA/cm/sup 2/ for 1 hr was best for biosensor fabrication. Porous silicon chips were functionalized using silanization and antibodies immobilized to the porous surface. Chemiluminescence-based assay was used to assess the functionalization of the biosensor. Light emission, for the silanized porous silicon biosensor chip with Salmonella was 10-fold greater than that of the control and non-silanized porous silicon with Salmonella. Higher light emission was observed for porous silanized biosensor with Salmonella compared to that of the non-porous chip. Porous silicon-based biosensor was fabricated and functionalized to successfully detect Salmonella.","PeriodicalId":369277,"journal":{"name":"Proceedings of IEEE Sensors 2003 (IEEE Cat. No.03CH37498)","volume":"64 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2003-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"9","resultStr":"{\"title\":\"Fabrication of porous silicon-based biosensor\",\"authors\":\"F. Mathew, E. Alocilja\",\"doi\":\"10.1109/ICSENS.2003.1278945\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"A porous silicon-based biosensor for detection of bacteria was fabricated. Silicon (0.01 ohm-cm, p-type) was anodized electrochemically in a hydrofluoric acid solution containing electrochemical cell using different current and time conditions ranging from 100 mA/cm2 for 30 sec to 2 mA/cm for 1 hr to produce sponge like porous layer of silicon. Anodizing conditions of 5 mA/cm/sup 2/ for 1 hr was best for biosensor fabrication. Porous silicon chips were functionalized using silanization and antibodies immobilized to the porous surface. Chemiluminescence-based assay was used to assess the functionalization of the biosensor. Light emission, for the silanized porous silicon biosensor chip with Salmonella was 10-fold greater than that of the control and non-silanized porous silicon with Salmonella. Higher light emission was observed for porous silanized biosensor with Salmonella compared to that of the non-porous chip. Porous silicon-based biosensor was fabricated and functionalized to successfully detect Salmonella.\",\"PeriodicalId\":369277,\"journal\":{\"name\":\"Proceedings of IEEE Sensors 2003 (IEEE Cat. No.03CH37498)\",\"volume\":\"64 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2003-10-22\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"9\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Proceedings of IEEE Sensors 2003 (IEEE Cat. No.03CH37498)\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1109/ICSENS.2003.1278945\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Proceedings of IEEE Sensors 2003 (IEEE Cat. No.03CH37498)","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1109/ICSENS.2003.1278945","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
A porous silicon-based biosensor for detection of bacteria was fabricated. Silicon (0.01 ohm-cm, p-type) was anodized electrochemically in a hydrofluoric acid solution containing electrochemical cell using different current and time conditions ranging from 100 mA/cm2 for 30 sec to 2 mA/cm for 1 hr to produce sponge like porous layer of silicon. Anodizing conditions of 5 mA/cm/sup 2/ for 1 hr was best for biosensor fabrication. Porous silicon chips were functionalized using silanization and antibodies immobilized to the porous surface. Chemiluminescence-based assay was used to assess the functionalization of the biosensor. Light emission, for the silanized porous silicon biosensor chip with Salmonella was 10-fold greater than that of the control and non-silanized porous silicon with Salmonella. Higher light emission was observed for porous silanized biosensor with Salmonella compared to that of the non-porous chip. Porous silicon-based biosensor was fabricated and functionalized to successfully detect Salmonella.
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