聚己内酯纳米纤维支架下人间充质干细胞向神经细胞分化的研究

Seyedeh Sara Karimian, S. Kaviani, M. Soleimani
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引用次数: 0

摘要

人间充质干细胞(hMSC)在纳米支架上分化为神经细胞是一种很有前途的生物异材料细胞移植治疗神经系统损伤的方法。hMSC的多电位特性已在各种组织工程研究中被发现。本研究显示了hMSC在3D和2D环境下的体外发育和神经分化。本研究使用的三维环境是纳米纤维聚己内酯(PCL)。研究了间充质干细胞(MSCs)在随机聚己内酯(PCL)纳米纤维支架和组织板上向神经细胞的分化潜能。研究证明,细胞外纳米纤维基质与体内细胞相互作用,对细胞具有机械维护作用,并在控制细胞行为方面发挥功能作用。干细胞正在成为组织工程和再生医学发展的基本工具。采用扫描电子显微镜(SEM)对PCL进行了表征。在DMDM/F12中加入维甲酸、表皮生长因子(EGF)、成纤维细胞生长因子(FGF-2)、Ibmx等神经诱导剂,将MSCs分化为神经细胞。扫描电镜和光学显微镜观察间充质细胞在PCL纳米纤维支架上的繁殖和神经形态。纳米纤维支架上分化的间充质细胞表达的神经基因标记包括;β-微管蛋白III和Map2在第14天。我们的研究表明,从hMSCs分化的神经细胞在纳米支架上的潜在用途是改善神经细胞。这项研究于2011年进行。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Evaluation of Human Mesenchymal Stem Cells Differentiation to Neural Cells on Polycaprolactone Nanofiber Scaffolds
: Differentiation of human mesenchymal stem cells (hMSC) to neural cells on Nano-scaffolds is a promising method for the treatment of the damaged nervous system through bionanomaterial-cell transplantation. The hMSC’s multipotential features have been discovered in various tissue engineering researches. This investigation shows the in-vitro development and neural differentiation of hMSC in 3D and 2D environments. The 3D environment which used in this study is nanofibrous polycaprolactone (PCL). The differentiation potential of mesenchymal stem cells (MSCs) to neural cells, on the random polycaprolactone (PCL) nanofibrous scaffolds, and tissue plate was examined. Researches have proved that interaction of extracellular nanofibrous matrix with in-vivo cells, gives mechanical maintenance to the cells and plays a functional role in the control of cellular behaviour. Stem cells are developing as a fundamental tool in the evolution of tissue engineering and regenerative medication. PCL characterization was determined employing scanning electron microscopy (SEM). Agents like, retinoic acid, epidermal growth factor (EGF), fibroblast growth factor (FGF-2), and Ibmx, which they are neural inducing agents, added in DMDM/F12 to differentiate MSCs to neural cells. Reproduction of mesenchymal cells on PCL nanofibrous scaffolds and neural morphology revealed through a scanning electron microscope (SEM) and optical microscope outcomes. The differentiated mesenchymal cells on nanofibrous scaffolds express neural gene markers including; β- tubulin III and Map2 on the day of 14. Our investigation recommends the potential usage of differentiated neural cells from hMSCs on Nano-scaffolds toward the improvement of neural cells. This study conducted in 2011.
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