Selwyn Daniel, Lorraine Malkowitz, Hsuei-Chin Wang, Bernard Beer, Arthur J. Blume, M.Reza Ziai
{"title":"筛选钾通道调节剂高通量86-铷外排试验在96孔微量滴度板","authors":"Selwyn Daniel, Lorraine Malkowitz, Hsuei-Chin Wang, Bernard Beer, Arthur J. Blume, M.Reza Ziai","doi":"10.1016/0160-5402(91)90009-T","DOIUrl":null,"url":null,"abstract":"<div><p>A rapid and sensitive <sup>86</sup>Rb efflux assay to detect chemical compounds capable of modulating the ATP-dependent potassium (K<sub>ATP</sub>) channel is described. This assay, which is performed in a 96-well microtiter plate, utilizes a substrate adherent cell line as the target, requires a small amount of <sup>86</sup>Rb as the tracer, and is a suitable system for performing the biochemical and pharmacological characterization of the K<sub>ATP</sub>-channel and its activators. Because this assay is amenable to automation, it presents a useful means for high-volume screening of chemical compounds on a routine basis.</p></div>","PeriodicalId":16819,"journal":{"name":"Journal of pharmacological methods","volume":"25 3","pages":"Pages 185-193"},"PeriodicalIF":0.0000,"publicationDate":"1991-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0160-5402(91)90009-T","citationCount":"20","resultStr":"{\"title\":\"Screening for potassium channel modulators by a high through-put 86-rubidium efflux assay in a 96-well microtiter plate\",\"authors\":\"Selwyn Daniel, Lorraine Malkowitz, Hsuei-Chin Wang, Bernard Beer, Arthur J. Blume, M.Reza Ziai\",\"doi\":\"10.1016/0160-5402(91)90009-T\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>A rapid and sensitive <sup>86</sup>Rb efflux assay to detect chemical compounds capable of modulating the ATP-dependent potassium (K<sub>ATP</sub>) channel is described. This assay, which is performed in a 96-well microtiter plate, utilizes a substrate adherent cell line as the target, requires a small amount of <sup>86</sup>Rb as the tracer, and is a suitable system for performing the biochemical and pharmacological characterization of the K<sub>ATP</sub>-channel and its activators. Because this assay is amenable to automation, it presents a useful means for high-volume screening of chemical compounds on a routine basis.</p></div>\",\"PeriodicalId\":16819,\"journal\":{\"name\":\"Journal of pharmacological methods\",\"volume\":\"25 3\",\"pages\":\"Pages 185-193\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1991-05-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/0160-5402(91)90009-T\",\"citationCount\":\"20\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of pharmacological methods\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/016054029190009T\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of pharmacological methods","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/016054029190009T","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Screening for potassium channel modulators by a high through-put 86-rubidium efflux assay in a 96-well microtiter plate
A rapid and sensitive 86Rb efflux assay to detect chemical compounds capable of modulating the ATP-dependent potassium (KATP) channel is described. This assay, which is performed in a 96-well microtiter plate, utilizes a substrate adherent cell line as the target, requires a small amount of 86Rb as the tracer, and is a suitable system for performing the biochemical and pharmacological characterization of the KATP-channel and its activators. Because this assay is amenable to automation, it presents a useful means for high-volume screening of chemical compounds on a routine basis.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
