The influence of oligoribonucleotide complexes with D-mannitol on tumor formation in a murine model of B16 melanoma

One of the ways of effective treatment of melanoma is based on strategies to enhance the body's immune response. OligoribonucleotidesD-mannitol (ORNs-D-M) complexes possess immunotherapeutic actions due to the activation of immune responses. Adult male mice of a C57BL/6J line were injected once by the ORNs-D-M solution at concentrations of 1.4; 0.7; 0.35; 0.175 mg. In the group where animals received 1.4 mg of ORNs-D-M, the formation of the solid tumors was not observed; however, in the group with 0.7 mg dose, the average tumor volume was 97% lower than the non-drug group. Also, the mRNA expression levels of markers of T-cell counts CD3, CD4, CD8, and CD247 approached those of healthy animals. Immunosuppressive state was decreased which reflect in the increased expression of CD274, PDCD1, and IFNB1.