工艺参数对酸解和酶解红豆果皮发酵糖产率影响的研究

Onoh Ikechukwu Maxwell, Anho Lawrence Oghenerivwe, Egwuagu Onyekachi
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引用次数: 0

摘要

研究了盐酸、硫酸和纤维素酶对椰皮的酸解和酶解作用。纤维素酶是由黑曲霉(Aspergillus Niger)真菌分泌的。对底物的近似分析表明,椰皮是一种木质纤维素生物质,纤维素成分为48%。考察了时间、温度、酸浓度、pH等工艺参数对椰皮酸解和酶解中葡萄糖得率的影响。在30℃、pH 5条件下酶解3天后,葡萄糖的最大产率为44.5%。HCl酸水解在70℃、5% HCl条件下水解180 min,葡萄糖产率达到27.3%。H2S04水解葡萄糖的产率相对低于HCl,在70℃条件下最高产率为26.5%,H2SO4在180分钟后达到5%。此外,利用傅里叶变换红外光谱(FTIR)对椰皮葡萄糖和标准葡萄糖中存在的官能团进行了评价。FTIR结果显示两种糖的官能团相似。椰皮可用于生产葡萄糖,并可进一步发酵生产乙醇。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Study of the effect of process parameters on the yield of fermentable sugar from red cocoyam (Xanthosoma sagittifolium) peels via acid and enzyme hydrolysis
The aim of this work is to study the acid and enzymatic hydrolysis of cocoyam peels using HCl, H2S04 acids and cellulase enzyme. The cellulase was secreted from Aspergillus Niger (A. niger) fungi. The proximate analysis of the substrate showed that cocoyam peel is a lignocellulosic biomass with a cellulose composition of 48%. The effect of the process parameters (time, temperature, acid concentration and pH) on the yield of glucose in acid and enzymatic hydrolysis of the cocoyam peel was respectively investigated. Maximum glucose yield of 44.5% was obtained after 3 days of enzymatic hydrolysis at 30°C and pH 5. The HCl acid hydrolysis showed a maximum glucose yield of 27.3% at 70°C, 5% HCl after 180 minutes. The glucose yield in H2S04 hydrolysis was relatively lower than that of the HCl with a maximum yield of 26.5% at 70°C, 5% H2SO4 after 180 minutes. In addition to, the functional groups present in the glucose synthesized from cocoyam ground peels and the standard glucose were evaluated using Fourier Transformed Infrared (FTIR). The FTIR results showed similarities in the functional groups present in both sugars. Cocoyam peel can be used for the production of glucose and further fermentative process to produce ethanol.
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