Radiation-induced mutations in genetic enhancement and development of new crop varieties in black gram (Vigna mungo (L.) Hepper).

Abstract Black gram (Vigna mungo (L.) Hepper), popularly known as urdbean or mash or black gram, is a grain legume rich in protein (25-28%), widely cultivated in the Indian subcontinent and to a lesser extent in Thailand, Australia and other Asian and South Pacific countries. Genetic improvement in this crop is hindered due to the narrow genetic base. As genetic variability is a prerequisite for any crop improvement programme, induced mutations provide an important source for generating variability. Radiation (gamma, X-rays and neutron) induced mutants were identified for various morphological and biochemical traits, creating a pool of genetic variability. These mutants were used in a cross-breeding programme to develop high-yielding, disease-resistant varieties in black gram. The effective blend of mutation and recombination breeding at the Bhabha Atomic Research Centre has resulted in the release of five black gram varieties (TAU-1, TAU-2, TPU-4, TU94-2 and TU-40) by incorporating desirable traits like large seed, wider adaptability, resistance to disease and improved quality. These varieties have been developed from mutants directly or by using them in cross-breeding programmes. For example, a black gram variety, N0.55, was irradiated with gamma-rays and electron beams to obtain a large number of mutants. The large-seed mutants, UM-196 and UM-201, were used in cross-breeding with the elite cultivar T-9 for developing the high-yielding varieties TAU-1, TAU-2, TPU-4, TU94-2 and TU-40. TAU-1 has become the most popular variety in Maharashtra state, occupying the maximum area under black gram cultivation. Induced mutations will continue to play an increasing role in generating genetic variability for various traits as a major component of environmentally sustainable agriculture.