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Comm. Horticulturae Journal Pub Date : 2018-12-13 DOI:10.29244/CHJ.2.2.11-16

Yeni Nurmaghfiroh, Suryo Wiyono

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摘要

抽象的。棕榈疫霉(Phytophthora palmivora)引起的木瓜基茎腐病是印尼木瓜的重要病害。本研究旨在研究拮抗菌对番木瓜幼苗根腐病的防治效果。这项研究是在茂物农业大学热带园艺研究中心实验室进行的。拮抗菌株为蜡样芽孢杆菌G4、硬芽孢杆菌J8、K1、小假单胞菌P14和铜绿假单胞菌pa2。采用双培养法对各拮抗菌进行体外抗菌能力测定。在不接种病原菌的情况下，采用生物测定法研究了受试菌对木瓜幼苗生长的促进作用。在温室试验中，通过人工接种病原菌，对5种细菌防治根腐病的效果进行了试验研究。以10 5 cfu/ml的剂量施用拮抗菌作为种子播种和浇水。各拮抗菌的抑菌率为24.99 ~ 34.99%。蜡样芽孢杆菌G4和铜绿假单胞菌对木瓜幼苗生长有促进作用。关键词:生物防治，木瓜，芽孢杆菌，假单胞菌，疫霉

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Keefektifan Beberapa Isolat Bakteri Antagonis untuk Mengendalikan Penyakit Busuk Pangkal Batang pada Bibit Pepaya yang Disebabkan oleh Phytophthora Palmivora

ABSTRACT . Basal stem rot caused by Phytophthora palmivora .is an important disease of papaya in Indonesia. This research aimed to study the effectiveness of antagonistic bacteria to control root rot on papaya seedlings. This research was conducted in the Laboratory of Center for Tropical Horticulture Studies (PKHT), Bogor Agricultural University. Antagonistic bacterial isolates used were Bacillus cereus G4, Bacillus firmus J8, K1, Pseudomonas diminuta P14, and Pseudomonas aeruginosa PA 2 . Antibiosis ability of all antagonistic bacteria was tested in vitro, by using dual culture test. Bioassay without pathogen inoculation was also performed to study the effect of tested bacteria on growth promotion of papaya seedlings. Effectiveness of five bacteria against basal stem rot was tested in a green house experiment with artificial inoculation of pathogens. Antagonistic bacteria was applied as seed trestment and watering at the rate of 10 5 cfu/ml. All antagonistic bacteria isolates suppressed the disease at the rate of 24.99 – 34.99%. Bacillus cereus G4 and Pseudomonas aeruginosa was able to increase growth of papaya.seedlings. Keyword: biological control, papaya, Bacillus, Pseudomonas, Phytophthora

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Comm. Horticulturae Journal

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