T. Mélissa, Olvera Vázquez Maricarmen, Vieyra Mexicano Cinthya, G. Valeria, Orocio Alcántara Nahúm Elías, M. D. Carmen
{"title":"韦拉克鲁斯州查恰拉卡斯屏障Actopan河异养菌群的生理类群","authors":"T. Mélissa, Olvera Vázquez Maricarmen, Vieyra Mexicano Cinthya, G. Valeria, Orocio Alcántara Nahúm Elías, M. D. Carmen","doi":"10.14196/sjas.v6i1.2358","DOIUrl":null,"url":null,"abstract":"The aim of this study was to determine physiological groups of heterotrophic community bacteria in River Actopan mouth, Chachalacas, Veracruz, Mexico. Water samples were collected in sterile jars in three zones of river: zone A (UrsuloGalvan bridge), zone B (La Loma de San Rafael) and zone C (La Bocana). The water samples were analyzed through specific culture mediums for heterotrophic bacteria groups that degrade collagen, cellulose, chitin, starch and hydrocarbons. Species were identified by conventional biochemical tests, identification strips API 20E and 20NE and for the case of bacteria that degrade hydrocarbons it was used the sequencing of RNA gen 16S. The results indicate that in zone A, bacteria that use collagen and starch as source of carbon were most abundant (105.6 cfumL -1 and 100 cfumL -1 respectively), in zone B, collagen and chitin bacteria obtained 71.6 cfumL -1 and 60 cfumL -1 , respectively. While in zone C, higher values were shown in gelatinases and cellulose degradative bacteria with 109 cfumL -1 and 106 cfumL -1 respectively, although it was also observed that this zone has more abundance in oil degrading bacteria with respect to zone A and B.","PeriodicalId":210831,"journal":{"name":"Scientific Journal of Animal Science","volume":"1 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2017-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Physiological groups of heterotrophic community bacteria present at River Actopan, Chachalacas barrier, Veracruz\",\"authors\":\"T. Mélissa, Olvera Vázquez Maricarmen, Vieyra Mexicano Cinthya, G. Valeria, Orocio Alcántara Nahúm Elías, M. D. Carmen\",\"doi\":\"10.14196/sjas.v6i1.2358\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The aim of this study was to determine physiological groups of heterotrophic community bacteria in River Actopan mouth, Chachalacas, Veracruz, Mexico. Water samples were collected in sterile jars in three zones of river: zone A (UrsuloGalvan bridge), zone B (La Loma de San Rafael) and zone C (La Bocana). The water samples were analyzed through specific culture mediums for heterotrophic bacteria groups that degrade collagen, cellulose, chitin, starch and hydrocarbons. Species were identified by conventional biochemical tests, identification strips API 20E and 20NE and for the case of bacteria that degrade hydrocarbons it was used the sequencing of RNA gen 16S. The results indicate that in zone A, bacteria that use collagen and starch as source of carbon were most abundant (105.6 cfumL -1 and 100 cfumL -1 respectively), in zone B, collagen and chitin bacteria obtained 71.6 cfumL -1 and 60 cfumL -1 , respectively. While in zone C, higher values were shown in gelatinases and cellulose degradative bacteria with 109 cfumL -1 and 106 cfumL -1 respectively, although it was also observed that this zone has more abundance in oil degrading bacteria with respect to zone A and B.\",\"PeriodicalId\":210831,\"journal\":{\"name\":\"Scientific Journal of Animal Science\",\"volume\":\"1 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2017-01-22\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Scientific Journal of Animal Science\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.14196/sjas.v6i1.2358\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Scientific Journal of Animal Science","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.14196/sjas.v6i1.2358","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
摘要
本研究的目的是确定墨西哥韦拉克鲁斯察查拉卡斯河Actopan口异养群落细菌的生理类群。在A区(UrsuloGalvan桥)、B区(La Loma de San Rafael)和C区(La Bocana)三个区域用无菌罐采集水样。通过特定培养基对水样进行异养细菌群的分析,这些异养细菌群可以降解胶原蛋白、纤维素、几丁质、淀粉和碳氢化合物。物种鉴定采用常规生化试验,鉴定条API 20E和20NE,降解烃类的细菌采用RNA gen 16S测序。结果表明,A区以胶原蛋白和淀粉为碳源的细菌数量最多(分别为105.6 cfumL -1和100 cfumL -1), B区以胶原蛋白和甲壳素为碳源的细菌数量分别为71.6 cfumL -1和60 cfumL -1。而在C区,明胶酶和纤维素降解细菌的cfumL -1的丰度较高,分别为109 cfumL -1和106 cfumL -1,尽管也观察到该区域的油降解细菌的丰度高于A区和B区。
Physiological groups of heterotrophic community bacteria present at River Actopan, Chachalacas barrier, Veracruz
The aim of this study was to determine physiological groups of heterotrophic community bacteria in River Actopan mouth, Chachalacas, Veracruz, Mexico. Water samples were collected in sterile jars in three zones of river: zone A (UrsuloGalvan bridge), zone B (La Loma de San Rafael) and zone C (La Bocana). The water samples were analyzed through specific culture mediums for heterotrophic bacteria groups that degrade collagen, cellulose, chitin, starch and hydrocarbons. Species were identified by conventional biochemical tests, identification strips API 20E and 20NE and for the case of bacteria that degrade hydrocarbons it was used the sequencing of RNA gen 16S. The results indicate that in zone A, bacteria that use collagen and starch as source of carbon were most abundant (105.6 cfumL -1 and 100 cfumL -1 respectively), in zone B, collagen and chitin bacteria obtained 71.6 cfumL -1 and 60 cfumL -1 , respectively. While in zone C, higher values were shown in gelatinases and cellulose degradative bacteria with 109 cfumL -1 and 106 cfumL -1 respectively, although it was also observed that this zone has more abundance in oil degrading bacteria with respect to zone A and B.