{"title":"高效液相色谱-二极管阵列检测(HPLC-DAD)测定动物饲料中的半胱胺","authors":"P. H. Nguyen","doi":"10.32508/STDJ.V21I2.427","DOIUrl":null,"url":null,"abstract":"A HPLC-DAD method for the determination of cysteamine supplementation in commercial animal feeds was developed. Samples were extracted with a mixture of 0.5 % hydrochloric acid – acetonitrile (90:10, v/v), matrix interferences were removed with a C18 cartridge, cysteamine was derivatized using 5,5'-dithiobis-(2-nitrobenzoic) acid (DTNB) as Ellman's reagent targeting to the thiol group in the molecule. Quantification of cysteamine was performed on a c18 column with DAD at 323 nm. The developed method had LOD of 1.1 mg/l, good linearity of the calibration curve (R2 ≥ 0.9998), high recoveries (> 92 %), and high reproducibility (RSD < 2.0%).","PeriodicalId":285953,"journal":{"name":"Science and Technology Development Journal","volume":"4 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2018-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"2","resultStr":"{\"title\":\"Determination of cysteamine in animal feeds by high performance liquid chromatography with diode-array detection (HPLC-DAD)\",\"authors\":\"P. H. Nguyen\",\"doi\":\"10.32508/STDJ.V21I2.427\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"A HPLC-DAD method for the determination of cysteamine supplementation in commercial animal feeds was developed. Samples were extracted with a mixture of 0.5 % hydrochloric acid – acetonitrile (90:10, v/v), matrix interferences were removed with a C18 cartridge, cysteamine was derivatized using 5,5'-dithiobis-(2-nitrobenzoic) acid (DTNB) as Ellman's reagent targeting to the thiol group in the molecule. Quantification of cysteamine was performed on a c18 column with DAD at 323 nm. The developed method had LOD of 1.1 mg/l, good linearity of the calibration curve (R2 ≥ 0.9998), high recoveries (> 92 %), and high reproducibility (RSD < 2.0%).\",\"PeriodicalId\":285953,\"journal\":{\"name\":\"Science and Technology Development Journal\",\"volume\":\"4 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2018-09-10\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"2\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Science and Technology Development Journal\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.32508/STDJ.V21I2.427\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Science and Technology Development Journal","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.32508/STDJ.V21I2.427","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Determination of cysteamine in animal feeds by high performance liquid chromatography with diode-array detection (HPLC-DAD)
A HPLC-DAD method for the determination of cysteamine supplementation in commercial animal feeds was developed. Samples were extracted with a mixture of 0.5 % hydrochloric acid – acetonitrile (90:10, v/v), matrix interferences were removed with a C18 cartridge, cysteamine was derivatized using 5,5'-dithiobis-(2-nitrobenzoic) acid (DTNB) as Ellman's reagent targeting to the thiol group in the molecule. Quantification of cysteamine was performed on a c18 column with DAD at 323 nm. The developed method had LOD of 1.1 mg/l, good linearity of the calibration curve (R2 ≥ 0.9998), high recoveries (> 92 %), and high reproducibility (RSD < 2.0%).
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