{"title":"多光子显微镜","authors":"W. Denk","doi":"10.1002/9781118696736.ch22","DOIUrl":null,"url":null,"abstract":"observation of and experimentation on intact tissue. There, in particular, high-resolution optical microscopy benefitsfram the inherent advantages of multi-photonfluorescence excitation Several cases will be presented from a number of different tissues and organisms, where multi-photon excited laser scanningfluorescence microscopy has been an essential experimental tool Those examples include the discove y of biochemical coincidence detection in synaptic spines and the clarification of the underlying mechanism, the observation of sensory evoked dendritic signaling in intact animals and the observation of light induced calcium signals in the intact retina. Recently ajiber coupled two-photon microscopy has been developed that allows imaging in moving animals.","PeriodicalId":268634,"journal":{"name":"Understanding Light Microscopy","volume":"5 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2019-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Multi-Photon Microscopy\",\"authors\":\"W. Denk\",\"doi\":\"10.1002/9781118696736.ch22\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"observation of and experimentation on intact tissue. There, in particular, high-resolution optical microscopy benefitsfram the inherent advantages of multi-photonfluorescence excitation Several cases will be presented from a number of different tissues and organisms, where multi-photon excited laser scanningfluorescence microscopy has been an essential experimental tool Those examples include the discove y of biochemical coincidence detection in synaptic spines and the clarification of the underlying mechanism, the observation of sensory evoked dendritic signaling in intact animals and the observation of light induced calcium signals in the intact retina. Recently ajiber coupled two-photon microscopy has been developed that allows imaging in moving animals.\",\"PeriodicalId\":268634,\"journal\":{\"name\":\"Understanding Light Microscopy\",\"volume\":\"5 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2019-03-13\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Understanding Light Microscopy\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1002/9781118696736.ch22\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Understanding Light Microscopy","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1002/9781118696736.ch22","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
observation of and experimentation on intact tissue. There, in particular, high-resolution optical microscopy benefitsfram the inherent advantages of multi-photonfluorescence excitation Several cases will be presented from a number of different tissues and organisms, where multi-photon excited laser scanningfluorescence microscopy has been an essential experimental tool Those examples include the discove y of biochemical coincidence detection in synaptic spines and the clarification of the underlying mechanism, the observation of sensory evoked dendritic signaling in intact animals and the observation of light induced calcium signals in the intact retina. Recently ajiber coupled two-photon microscopy has been developed that allows imaging in moving animals.
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