坦桑尼亚北部鸡、人、啮齿动物和土壤中分离的多重耐药金黄色葡萄球菌抗生素耐药性和毒力基因的多重PCR检测

V. S. Sonola, A. Katakweba, Gerald Misinzo, M. I. Matee
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引用次数: 0

摘要

金黄色葡萄球菌(金黄色葡萄球菌)是一种具有公共卫生和兽医重要性的人畜共患病原体。采用多重聚合酶链反应(multiple - PCR)技术,对从人(n=17)、鸡(n=14)、啮齿动物(n=13)和土壤(n=13)分离的57株多重耐药(MDR)金黄色葡萄球菌进行了抗生素耐药基因(ARGs)和毒力基因(VGs)检测。总体而言,ARGs分布显示,tetK在18/57 (31.6%),mecA在16/57 (28.1%),tetL在5/57 (8.9%),ermC在1/57(1.8%),而ermA和tetM未检出。对于VGs, clfB在6/57 (10.5%),coa在8/57 (14.0%),clfA在3/57 (5.3%),hlg在1/57 (1.8%),ebpS在2/57 (3.5%),fnbB在2/57 (3.5%),luk-PV在6/57 (10.5%),tst在1/57(1.8%)。耐药基因(tetK和mecA)和毒力决定因素(clfB、coa和luk-PV)在所有样本来源中都是常见的,而test、hlg和fnbB分别是人类、鸡和啮齿动物分离株所特有的。红霉素表型耐药结果与ermC (r=0.42)、tetL (r=0.98)和mecA (r=0.51)相关,四环素耐药与tetL (r=1.00)和mecA (r=0.57)基因相关,甲氧西林耐药与mecA (r=0.55)和tetL (r=0.98)基因相关。ARG (ermC)与VGs呈正相关;clfA (r=0.57)、hlg (r=1.00)和clfB (r=0.43), tetK和clfB之间(r=0.39);tetK和coa基因(r=0.36)。主成分分析(PCA)表明,tetL、ermC和mecA分别对四环素、红霉素和甲氧西林耐药起作用。从人、鸡、啮齿动物和土壤样本分离的耐多药金黄色葡萄球菌中广泛存在耐药性和毒力基因(通常是组合存在),需要全面的“一种健康”干预措施。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Multiplex PCR detection of antibiotic resistance and virulence genes in multidrug-resistant Staphylococcus aureus isolated from chickens, humans, rodents, and soil in Northern Tanzania
Staphylococcus aureus (S. aureus) is a zoonotic pathogen with public health and veterinary importance. We investigated the presence of antibiotic resistance genes (ARGs) and virulence genes (VGs) in 57 multidrug-resistant (MDR) S. aureus isolated from humans (n=17), chickens (n=14), rodents (n=13), and soil (n=13) using multiplex PCR. Overall, the distribution of ARGs revealed that the tetK was found in 18/57 (31.6%), mecA in 16/57 (28.1%), tetL in 5/57 (8.9%), and ermC in 1/57 (1.8%), while ermA and tetM were not detected. For VGs, the clfB was found in 6/57 (10.5 %), coa in 8/57 (14.0%), clfA in 3/57 (5.3%), hlg in 1/57 (1.8%), ebpS in 2/57 (3.5%), fnbB in 2/57 (3.5%), luk-PV in 6/57 (10.5%) and tst in 1/57 (1.8%). Resistance genes (tetK and mecA) and virulence determinants (clfB, coa, and luk-PV) were common in all sample sources, while tst, hlg, and fnbB were specific to human, chicken, and rodent isolates, respectively. Erythromycin phenotypic resistance results correlated with the presence of ermC (r=0.42), tetL (r=0.98), and mecA (r=0.51), while tetracycline resistance correlated with tetL (r=1.00) and mecA (r=0.57) genes and methicillin resistance correlated with mecA (r=0.55) and tetL (r=0.98) genes. Positive correlations were noted between ARG (ermC) and VGs; clfA (r=0.57), hlg (r=1.00), and clfB (r=0.43), and between tetK and clfB (r=0.39); tetK and coa (r=0.36) genes. Principal component analysis (PCA) shows that tetL, ermC, and mecA contributed to tetracycline, erythromycin, and methicillin resistance, respectively. The widespread presence of resistance and virulence genes, often in combination, among MDR S. aureus in isolates from humans, chicken, rodents, and soil samples require comprehensive One-Health interventions.
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