Maria F. T. Leyn, Henderiana L. L. Belli, W. M. Nalley, P. Kune, T. Hine
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摘要

本研究旨在研究在三卵黄稀释液中添加不同浓度的火龙果皮提取物(DFPE)对白头翁精子质量的影响。采用人工阴道法采集体况正常、生殖器官正常的3岁白斑公山羊的精液,每周2次。经宏观和微观评价,精子活动力> 70%、浓度> 800 × 106 mL-1、异常率< 15%的优质精液,用TEY + DFPE稀释,浓度分别为0% (P0)、2% (P1)、4% (P2)、6% (P3)、8% (P4)、10% (P5)。将活力≥70%的精液保存在3-5℃的温度下。每24小时进行一次精子质量评价,直到精子活力下降到40%。结果表明,保存第1 ~ 5天,TEY + 6% DFPE稀释液(P3)中保存的精子活力和活力高于其他处理(P< 0.05)。保存5 d前精子异常率低于6%,但与对照组相比差异有统计学意义(P< 0.05)。综上所述,在TEY中添加高达6%的DFPE可减轻体外保存过程中白头山山羊精子质量的下降。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
KUALITAS SPERMATOZOA KAMBING BLIGON DALAM PENGENCER TRIS-KUNING TELUR DENGAN PENAMBAHAN BERBAGAI LEVEL EKSTRAK KULIT BUAH NAGA (Spermatozoa quality of bligon goat in tris - egg yolk diluent added with various levels of dragon fruit peel extract)
The purpose of this study was to examine the effect of adding various concentrations of dragon fruit peel extract (DFPE) in Tris-egg yolk diluent (TEY) on the quality of bligon goat spermatozoa. Semen was collected twice a week using the artificial vaginal method from three year old bligon male goats with normal body conditions and reproductive organs. After the macroscopic and microscopic evaluation, good quality semen with a sperm motility of > 70%, a concentration of > 800 x 106 mL-1, and abnormality of < 15 %, was diluted with TEY + DFPE at a concentration of 0% (P0), 2% (P1), 4% (P2), 6% (P3), 8% (P4), dan 10% (P5). The semen which still had a motility ≥ 70% was then stored at a temperature of 3-5oC. Evaluation of the quality of spermatozoa  was carried out every 24 hours until the motility decrease to 40%. The results showed that from day 1 to day 5 of preservation, spermatozoa preserved in TEY + 6% DFPE diluent (P3) had higher motility and viability (P<0,05) than other treatments. The abnormalities of spermatozoa until the 5 day of preservation were below 6%, but showed a significant difference (P<0,05) between treatments that received DFPE suplemention with control (P0). It was concluded that the addition of DFPE in TEY up to 6% could reduce the decrease in sperm quality of bligon goats during in vitro preservation.
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