Determination of Cytotoxic, Anti-acetylcholinesterase and Antioxidant Activity of Some Medicinal Artemisia spp.

The study aims to investigated the antioxidant activities, phenolic compounds, acetylcholinesterase enzyme inhbition and cytotoxic effects of two different of Artemisia spp. (Artemisia dracunculus L. and Artemisia dracunculoides Pursh) cultivated in Turkey organically, for the first time. Total phenolic, total flavonoid contents of the plants determined spectrophotometrically while antioxidant activity DPPH, CUPRAC, and FRAP was determined using colorimetric method. And also acetylcholinesterase enzyme inhibition activity and anti-cancer activity in-vitro aganist human melanoma (VMM917, CRL-3232), lung carcinoma (A549, CCL-185) and, normal human fibroblast (hGF, PCS-201-018) cells were studied. Total phenolic (225-324 mgGAE/g sample) and total flavonoid contents (0.066-0.085 mgQAE/g sample), antioxidant activity (DPPH (1.371-1.655 mg/mL), CUPRAC (0.246-0.344 µM CTEAC) and FRAP (462.133-726.661 µM CTEAC)). A. dracunculus and A. dracunculoides extracts inhibited 40.09±0.65%, and 39.48±3.68% of acetylcholinesterase activity. It was determined that demonstrated the selective effect of A. dracunculus and A. dracunculoides on the cytotoxicity of A549 and hGF cells.