Green Synthesis of Copper Nanoparticles using Mitragyna parvifolia Plant Bark Extract and Its Antimicrobial Study

In this study, Mitragyna parvifolia plant bark used to prepare aqueous extract which provides cost-effective, eco-friendly process, less time consuming, an environmentally benign, easy and proficient way for the synthesis of copper nanoparticles. Mitragyna parvifolia plant bark was collected from Virpur hills forest area. The Mitragyna parvifolia plant bark extract was prepared with deionised water and used for the green synthesis of copper nanoparticles. The colour change of the solution dark brown from pale yellow colour confirms the formation of copper nanoparticles. The green synthesised copper nanoparticles were characterised by UV-visible spectroscopy, FT-IR, XRD, SEM, TEM and their antimicrobial activity was also investigated. UV-visible spectral result confirmed the reduction of copper sulphate to copper nanoparticles. FTIR analysis also supported for the formation of copper nanoparticles. Crystallinity of Cu NPs was find out by XRD study and the morphology of the particles was analysed with the help of scanning electron microscopy and found spherical in nature. The antibacterial activity experiment done against Escherichia coli, gram-negative and Bacillus subtilis, gram-positive bacteria by agar well method and the maximum zone of inhibition was higher in gram-positive bacteria compared to gram-negative bacteria. The green synthesised copper nanoparticles proved to be potential candidates for medical application where the antimicrobial activity is highly essential.