SMCC-7721细胞的AFAM定量成像与分析

2016 IEEE International Conference on Manipulation, Manufacturing and Measurement on the Nanoscale (3M-NANO) Pub Date : 2016-07-01 DOI:10.1109/3M-NANO.2016.7824987

Zuobin Wang, Yujing Zhao, Xinyue Wang, Yang Yang, Yan Liu

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引用次数: 0

摘要

本文提出了一种利用原子力声显微镜(AFAM)对SMCC-7721细胞进行定量成像和分析的方法。本研究利用AFAM对SMCC-7721细胞的表面、胞内结构和弹性作图进行了研究，获得了细胞的形貌和声学图像。实验中，SMCC-7721细胞分别用4%多聚甲醛(PFA)处理，胸腺嘧啶(TdR)处理和未处理。对影像学结果进行比较分析。定量分析结果表明，AFAM是测量细胞表面、胞内结构和弹性性能的有效工具。

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Quantitative imaging and analysis of SMCC-7721 cells Using AFAM

This paper presents a method for the quantitative imaging and analysis of SMCC-7721 cells using an atomic force acoustic microscope (AFAM). In this work, an AFAM was used to study the surface, intracellular structure and elasticity mapping of SMCC-7721 cells, and both topographic and acoustic images of the cells were obtained. In the experiment, the SMCC-7721 cells were treated with 4% paraformaldehyde (PFA), and untreated and treated with Thymidine (TdR). The imaging results were compared and analyzed. The quantitative analysis result indicates that the AFAM is a useful tool to measure the surface, intracellular structure and the elasticity property of the cells.

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2016 IEEE International Conference on Manipulation, Manufacturing and Measurement on the Nanoscale (3M-NANO)

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