{"title":"大肠杆菌Rec突变体缺乏Rec BC (D)复合物亚基。","authors":"E Tenke, G Bánfalvi","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>The inactivation of rec BC (D) DNase upon chromatography on DEAE-cellulose was observed. Simultaneously DNA-stimulated ATPases (I and II) and DNase activities on single- and double-stranded DNA substrates were measured in Escherichia coli rec+ and rec- cell extracts. Normal levels of ATPase I and II were detected in rec+ cells. Rec A- cells were lacking DNA dependent ATPase I, while rec B single and rec BC double mutants were defective in DNA dependent ATPase II, the second major enzyme of this type. Rec B and C mutations did not change DNase activities. Rec A mutation significantly increased DNase activity on linear single-stranded substrate.</p>","PeriodicalId":77479,"journal":{"name":"Acta biochimica et biophysica Hungarica","volume":"25 1-2","pages":"101-9"},"PeriodicalIF":0.0000,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Rec mutants of Escherichia coli deficient in subunits of rec BC (D) complex.\",\"authors\":\"E Tenke, G Bánfalvi\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>The inactivation of rec BC (D) DNase upon chromatography on DEAE-cellulose was observed. Simultaneously DNA-stimulated ATPases (I and II) and DNase activities on single- and double-stranded DNA substrates were measured in Escherichia coli rec+ and rec- cell extracts. Normal levels of ATPase I and II were detected in rec+ cells. Rec A- cells were lacking DNA dependent ATPase I, while rec B single and rec BC double mutants were defective in DNA dependent ATPase II, the second major enzyme of this type. Rec B and C mutations did not change DNase activities. Rec A mutation significantly increased DNase activity on linear single-stranded substrate.</p>\",\"PeriodicalId\":77479,\"journal\":{\"name\":\"Acta biochimica et biophysica Hungarica\",\"volume\":\"25 1-2\",\"pages\":\"101-9\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1990-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Acta biochimica et biophysica Hungarica\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Acta biochimica et biophysica Hungarica","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
摘要
在deae -纤维素层析上观察了rec BC (D) dna酶的失活。同时测定了大肠杆菌rec+和rec-细胞提取物中DNA刺激的atp酶(I和II)和DNA酶在单链和双链DNA底物上的活性。在rec+细胞中检测到正常水平的atp酶I和II。Rec A-细胞缺乏DNA依赖性atp酶I，而Rec B单突变体和Rec BC双突变体缺乏DNA依赖性atp酶II，这是该类型的第二大酶。Rec B和C突变不改变dna酶活性。Rec A突变显著增加了线性单链底物上的dna酶活性。
Rec mutants of Escherichia coli deficient in subunits of rec BC (D) complex.
The inactivation of rec BC (D) DNase upon chromatography on DEAE-cellulose was observed. Simultaneously DNA-stimulated ATPases (I and II) and DNase activities on single- and double-stranded DNA substrates were measured in Escherichia coli rec+ and rec- cell extracts. Normal levels of ATPase I and II were detected in rec+ cells. Rec A- cells were lacking DNA dependent ATPase I, while rec B single and rec BC double mutants were defective in DNA dependent ATPase II, the second major enzyme of this type. Rec B and C mutations did not change DNase activities. Rec A mutation significantly increased DNase activity on linear single-stranded substrate.
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