胰腺癌患者腹水中胰腺癌相关抗原的部分特征及临床评价

M Saito, T Shimano, T Kobayashi, H Inaji, N Matsuura, S Masutani, T Mori
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摘要

利用已建立的杂交瘤细胞制备胰腺癌相关抗原(PCAAp)单克隆抗体。选择了两种单克隆抗体3F1和3B6,在免疫组织学上发现这两种单克隆抗体对分化良好的胰腺癌组织中的癌细胞和导管内黏液样产物有强烈的反应,但对胃癌、结直肠癌和其他癌症反应微弱,如果有的话,对正常成人或胎儿胰腺组织则完全没有反应。PCAAp通常在正常结肠黏膜(PCAAc)中表达,但这两种单克隆抗体与正常结肠黏膜几乎没有反应。采用夹心酶免疫分析法测定循环PCAAp。对32名正常人和271例患者进行了调查,其中恶性疾病210例,良性疾病61例。32名正常受试者中PCAAp水平的临界值为2.06微克/毫升,即平均值+ 2SD,而在72%的胰腺癌患者、65%的胆管癌患者、60%的肝癌患者、0- 30%的其他恶性疾病患者和10%的良性肝胆疾病患者中,PCAAp水平高于此值。研究了分离的PCAAp和PCAAc对各种酶和化学试剂的敏感性。本研究结果提示PCAAp检测可能有助于胰腺癌的诊断。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The partial characterization and clinical evaluation of pancreas cancer-associated antigen from the ascites fluid of a patient with pancreatic cancer.

Monoclonal antibodies against pancreas cancer-associated antigen (PCAAp) were produced by established hybridoma cells. Two monoclonal antibodies, 3F1 and 3B6, were selected and these two monoclonal antibodies were found immunohistologically to react strongly with cancer cells and intraductal mucin-like products in well-differentiated pancreatic cancer tissues, but weakly, if at all, with gastric, colorectal and other cancers and at all not with normal adult or fetal pancreatic tissue. PCAAp is usually expressed in normal colonic mucosa (PCAAc), but the two monoclonal antibodies scarcely reacted with normal colonic mucosa. A sandwich enzyme immunoassay was developed to measure circulating PCAAp. Thirty-two normal subjects and 271 patients comprised of 210 with malignant disease and 61 with benign disease were surveyed. The cut-off value of PCAAp levels in the 32 normal subjects was 2.06 micrograms/ml, being the mean + 2SD, while PCAAp levels of more than this were observed in 72 per cent of the patients with pancreatic cancers, 65 per cent of those with bile duct cancers, 60 per cent of those with hepatomas, 0-30 per cent of those with other malignant diseases, and 10 per cent of those with benign hepatobiliary diseases. The susceptibility of isolated PCAAp and PCAAc to various enzymes and chemical reagents were also studied. The results of this study suggest that the assay for PCAAp might be useful in the diagnosis of pancreatic cancer.

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