{"title":"不同培养基生产纯黄曲霉毒素B1 (AFB1)的研究","authors":"A. G. Radhi, M. Adhab, H. Z. Hussein","doi":"10.1109/IEEECONF53624.2021.9668050","DOIUrl":null,"url":null,"abstract":"Aflatoxin B1 (AFB1) is a mycotoxin that is produced by Aspergillus flavus as a secondary metabolite. This study aimed to select the optimal medium to grow A. flavus to produce the highest concentration of AFB1. Solid-state fermentation of rice, corn, and a mixture of corn, sugar, peanut, and coconut (CSPC), and semisynthetic liquid culture of Yeast Extract sucrose Broth (YEB), Yeast Extract Sucrose Broth with Peptone (YEBP), and Czapek-Dox liquid Medium (Czp-D) were tested in this study. The AFB1 was extracted using the organic solvents method and purified through Thin Layer Chromatography (TLC). The concentration of AFB1 was then estimated using High-Performance Liquid Chromatography (HPLC). The results showed that the Czp-D medium had the potential to produce the highest concentration of AFB1, while the lowest concentration of AFB1 was gained when using the rice medium.","PeriodicalId":389608,"journal":{"name":"2021 Third International Sustainability and Resilience Conference: Climate Change","volume":"45 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2021-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Production of Pure Aflatoxin B1 (AFB1) Using Different Media\",\"authors\":\"A. G. Radhi, M. Adhab, H. Z. Hussein\",\"doi\":\"10.1109/IEEECONF53624.2021.9668050\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Aflatoxin B1 (AFB1) is a mycotoxin that is produced by Aspergillus flavus as a secondary metabolite. This study aimed to select the optimal medium to grow A. flavus to produce the highest concentration of AFB1. Solid-state fermentation of rice, corn, and a mixture of corn, sugar, peanut, and coconut (CSPC), and semisynthetic liquid culture of Yeast Extract sucrose Broth (YEB), Yeast Extract Sucrose Broth with Peptone (YEBP), and Czapek-Dox liquid Medium (Czp-D) were tested in this study. The AFB1 was extracted using the organic solvents method and purified through Thin Layer Chromatography (TLC). The concentration of AFB1 was then estimated using High-Performance Liquid Chromatography (HPLC). The results showed that the Czp-D medium had the potential to produce the highest concentration of AFB1, while the lowest concentration of AFB1 was gained when using the rice medium.\",\"PeriodicalId\":389608,\"journal\":{\"name\":\"2021 Third International Sustainability and Resilience Conference: Climate Change\",\"volume\":\"45 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2021-11-15\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"2021 Third International Sustainability and Resilience Conference: Climate Change\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1109/IEEECONF53624.2021.9668050\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"2021 Third International Sustainability and Resilience Conference: Climate Change","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1109/IEEECONF53624.2021.9668050","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Production of Pure Aflatoxin B1 (AFB1) Using Different Media
Aflatoxin B1 (AFB1) is a mycotoxin that is produced by Aspergillus flavus as a secondary metabolite. This study aimed to select the optimal medium to grow A. flavus to produce the highest concentration of AFB1. Solid-state fermentation of rice, corn, and a mixture of corn, sugar, peanut, and coconut (CSPC), and semisynthetic liquid culture of Yeast Extract sucrose Broth (YEB), Yeast Extract Sucrose Broth with Peptone (YEBP), and Czapek-Dox liquid Medium (Czp-D) were tested in this study. The AFB1 was extracted using the organic solvents method and purified through Thin Layer Chromatography (TLC). The concentration of AFB1 was then estimated using High-Performance Liquid Chromatography (HPLC). The results showed that the Czp-D medium had the potential to produce the highest concentration of AFB1, while the lowest concentration of AFB1 was gained when using the rice medium.
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