ABA和H2O2胁迫下小麦胚和胚乳特异性比较转录组分析

Saurabh Gupta, Utkarsh Raj, Himansu Kumar, Pritish Kumar Varadwaj, Adhyana Gupta
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引用次数: 6

摘要

小麦种子发芽直接影响小麦的产量和品质。胚和胚乳是小麦种子的两个主要组成部分,在种子萌发和随后的植物生长发育中起着至关重要的作用。在种子萌发过程中,系统的双向分子相互作用是主要组成部分,并受到非生物胁迫的影响。迄今为止,各种蛋白质组学和转录组学方法已被用于研究种子萌发过程对非生物胁迫的响应。在这项研究中,我们利用微阵列数据,利用胁迫系统生物学的方法研究了胚胎和胚乳对ABA和H2O2胁迫的顺从表达基因/转录本(DEGs)。进一步探讨了基因在不同胁迫下的作用,确定了基因在种子萌发、胚和胚乳过程中的生物学和分子功能。我们的研究结果揭示了种子萌发过程中胚和胚乳对ABA和H2O2处理的转录组学比较机制。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Embryo and endosperm specific comparative transcriptome analysis of triticum aestivum in response to ABA and H2O2 stress
Wheat seed germination directly affects wheat yield and quality. Embryo and endosperm are two main parts of wheat seed and plays crucial role in seed germination and subsequent plant growth and other development. In the process of seed germination a systematic bi-directional molecular interactions are major component and that got affected by the means of abiotic stresses. A variety of proteomic and transcriptomic approaches have been used so far to investigate seed germination process in response to abiotic stresses. In this study, we have investigated deferentially expressed genes/transcripts (DEGs) of embryo and endosperm in response to ABA and H2O2 stress by the means of stress systems biology approaches using microarray data. Further, role of DEGs in response to each stress was explored, which identify biological and molecular functions of genes during seed germination embryo and endosperm. Our results provide the insight into comparative transcriptomic mechanism of the embryo and endosperm in response to ABA and H2O2 treatment during seed germination process.
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