{"title":"光敏剂靶向:线粒体靶向光敏剂增强光动力效应和细胞凋亡","authors":"M. Jou","doi":"10.1109/APBP.2004.1412294","DOIUrl":null,"url":null,"abstract":"Using multiphoton laser scanning imaging microscopy the effects of subcellular localized PDT with the photosensitizer BPD-MA was studied in different cellular compartments including the mitochondria, the cytosol and the nucleus. Specifically, mitochondrial reactive oxygen species (mROS) and mitochondrial free calcium (mCa/sup 2+/) were measured simultaneously during PDT with BPD-MA induced mitochondrial dysfunction. Intriguingly, PDT with BPD-MA-induced mROS formation and mCa/sup 2+/ elevation as well as apoptotic death were found only when the mitochondrial compartment was illuminated. These data suggest that PDT with a mitochondria targeted photosensitizer can extensively enhance the efficacy of PDT via mROS and mCa/sup 2+/-mediated apoptotic mechanisms.","PeriodicalId":346624,"journal":{"name":"The Second Asian and Pacific Rim Symposium on Biophotonics, 2004. APBP 2004.","volume":"3 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2004-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Photosensitizer targeting: mitochondria-targeted photosensitizer enhances photodynamic effect and apoptosis\",\"authors\":\"M. Jou\",\"doi\":\"10.1109/APBP.2004.1412294\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Using multiphoton laser scanning imaging microscopy the effects of subcellular localized PDT with the photosensitizer BPD-MA was studied in different cellular compartments including the mitochondria, the cytosol and the nucleus. Specifically, mitochondrial reactive oxygen species (mROS) and mitochondrial free calcium (mCa/sup 2+/) were measured simultaneously during PDT with BPD-MA induced mitochondrial dysfunction. Intriguingly, PDT with BPD-MA-induced mROS formation and mCa/sup 2+/ elevation as well as apoptotic death were found only when the mitochondrial compartment was illuminated. These data suggest that PDT with a mitochondria targeted photosensitizer can extensively enhance the efficacy of PDT via mROS and mCa/sup 2+/-mediated apoptotic mechanisms.\",\"PeriodicalId\":346624,\"journal\":{\"name\":\"The Second Asian and Pacific Rim Symposium on Biophotonics, 2004. APBP 2004.\",\"volume\":\"3 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2004-12-15\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"The Second Asian and Pacific Rim Symposium on Biophotonics, 2004. APBP 2004.\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1109/APBP.2004.1412294\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"The Second Asian and Pacific Rim Symposium on Biophotonics, 2004. APBP 2004.","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1109/APBP.2004.1412294","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Photosensitizer targeting: mitochondria-targeted photosensitizer enhances photodynamic effect and apoptosis
Using multiphoton laser scanning imaging microscopy the effects of subcellular localized PDT with the photosensitizer BPD-MA was studied in different cellular compartments including the mitochondria, the cytosol and the nucleus. Specifically, mitochondrial reactive oxygen species (mROS) and mitochondrial free calcium (mCa/sup 2+/) were measured simultaneously during PDT with BPD-MA induced mitochondrial dysfunction. Intriguingly, PDT with BPD-MA-induced mROS formation and mCa/sup 2+/ elevation as well as apoptotic death were found only when the mitochondrial compartment was illuminated. These data suggest that PDT with a mitochondria targeted photosensitizer can extensively enhance the efficacy of PDT via mROS and mCa/sup 2+/-mediated apoptotic mechanisms.
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