{"title":"超声表征细胞培养基在25°C至50°C的温度范围内","authors":"S. A. López-Haro, J. L. Reyes, A. Vera, L. Leija","doi":"10.1109/PAHCE.2012.6233443","DOIUrl":null,"url":null,"abstract":"The aim of this work was the ultrasonic characterization of the cell culture medium used for Madin-Darby canine kidney (MDCK) cells. To design and build a special container for the speed-of-sound (SOS) temperature dependence measurement of the cell culture media was necessary. Pulse-echo technique and a step reflector were used to obtain the temperature dependence of SOS results. Water and cell culture media SOS are compared; both liquids behavior show an upward tendency in the temperature range studied. Culture media SOS results were fitted by using both a first and a second order curve; second order curve had better correlation coefficient than the other one. Speed-of-sound varies from 1510 m/s at 25°C to 1540 m/s at 50°C.","PeriodicalId":255935,"journal":{"name":"2012 Pan American Health Care Exchanges","volume":"43 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2012-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"2","resultStr":"{\"title\":\"Ultrasonic characterization of cell culture medium in the temperature range from 25°C to 50°C\",\"authors\":\"S. A. López-Haro, J. L. Reyes, A. Vera, L. Leija\",\"doi\":\"10.1109/PAHCE.2012.6233443\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The aim of this work was the ultrasonic characterization of the cell culture medium used for Madin-Darby canine kidney (MDCK) cells. To design and build a special container for the speed-of-sound (SOS) temperature dependence measurement of the cell culture media was necessary. Pulse-echo technique and a step reflector were used to obtain the temperature dependence of SOS results. Water and cell culture media SOS are compared; both liquids behavior show an upward tendency in the temperature range studied. Culture media SOS results were fitted by using both a first and a second order curve; second order curve had better correlation coefficient than the other one. Speed-of-sound varies from 1510 m/s at 25°C to 1540 m/s at 50°C.\",\"PeriodicalId\":255935,\"journal\":{\"name\":\"2012 Pan American Health Care Exchanges\",\"volume\":\"43 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2012-03-26\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"2\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"2012 Pan American Health Care Exchanges\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1109/PAHCE.2012.6233443\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"2012 Pan American Health Care Exchanges","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1109/PAHCE.2012.6233443","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Ultrasonic characterization of cell culture medium in the temperature range from 25°C to 50°C
The aim of this work was the ultrasonic characterization of the cell culture medium used for Madin-Darby canine kidney (MDCK) cells. To design and build a special container for the speed-of-sound (SOS) temperature dependence measurement of the cell culture media was necessary. Pulse-echo technique and a step reflector were used to obtain the temperature dependence of SOS results. Water and cell culture media SOS are compared; both liquids behavior show an upward tendency in the temperature range studied. Culture media SOS results were fitted by using both a first and a second order curve; second order curve had better correlation coefficient than the other one. Speed-of-sound varies from 1510 m/s at 25°C to 1540 m/s at 50°C.
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