A. M. Teles, Adenilde Nascimento Mouchreck, G. O. Everton, A. L. Abreu-Silva, K. Calabrese, Fernando Almeida-Souza
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引用次数: 1
摘要
野外有许多食源性病原体,它们被认为是发达国家和发展中国家严重公共卫生问题的原因。为了控制食品中存在的细菌,主要是对传统抗生素具有耐药性的病原体,诸如抗菌化合物等天然产物的使用一直在增加。本章旨在提供肉桂(Cinnamomum zeylanicum)、牛至(Origanum vulgare)、生姜(Zingiber officinale)、迷迭香(Rosmarinus officinalis)、大溪地柠檬(Citrus latifolia)和藏红花(Curcuma longa)精油的抗菌和抗氧化活性,并测定其化学成分。用Clevenger型装置对精油进行了加氢蒸馏提取,并对标准菌株大肠杆菌、铜绿假单胞菌和金黄色葡萄球菌进行了抑菌活性测定。采用ABTS[2,2-氮唑-(3-乙基苯并噻唑-6-磺酸)]法测定其抗氧化活性。精油是单萜和倍半萜的混合物。确定了zeylanicum对金黄色葡萄球菌的最佳最小抑制浓度。14 μ g mL−1的抗氧化活性抑制率为90.74%,ec50抑制率为90.74%。结果表明,所分析的精油具有良好的抑菌活性和抗氧化作用,能够满足控制食品中微生物的需要。
Comparative Analysis of the Chemical Composition, Antimicrobial and Antioxidant Activity of Essential Oils of Spices Used in the Food Industry in Brazil
There are many food-borne pathogens in the wild and they are considered the cause of serious public health problems in both developed and developing countries. The use of natural products, such as antimicrobial compounds, has been increasing, in an attempt to control bacteria present in foods, mainly pathogens resistant to conventional antibiotics. This chapter is intended to provide the antimicrobial and antioxidant activity of essential oils of Cinnamomum zeylanicum (cinnamon), Origanum vulgare (oregano), Zingiber officinale (ginger), Rosmarinus officinalis (rosemary), Citrus latifolia (tahiti lemon) and Curcuma longa (saffron) as well as to determinate its chemical composition. The oils had been extracted by hydrodistillation with a Clevenger type apparatus and the antimicrobial activity was performed against standard strains Escherichia coli , Pseudomonas aeruginosa and Staphylococcus aureus . The antioxidant activity was carried out using the ABTS [2,2-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid)] method. The essential oils presented a mixture of mono- and sesquiterpenes. The best minimum inhibitory concentration was determined to C. zeylanicum against S. aureus . O. vulgare antioxidant activity presented inhibition of 90.74% and EC 50 of 14 μ g mL − 1 . These results demonstrate that the essential oils analyzed presented efficient antibacterial activity and antioxidant action being able to satisfy the demand of use as control of microorganisms in the food.