{"title":"[肺炎支原体抗原的免疫生物学特征及其感染的诊断]。","authors":"A Ia Pustovar","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>The paper presents the developed variants of enzyme immunoassay (EIA) using two M. hyopneumoniae (R-14 strain) antigens obtained by biomass freezing and thawing and two Tween-dissolved antigens by gel chromatography. To identify the cultures isolated, an indirect immunofluorescence reaction was proposed by employing agar blocks. The developed methods made it possible to identify 23 bovine-isolated cultures as M. hyopneumoniae antigens. The latter were detected by EIA in diagnostically significant titers in serum and colostrum samples from 2 swine-breeding farms. A negative result was obtained in the respiratory mycoplasmosis-free farms.</p>","PeriodicalId":76796,"journal":{"name":"Vestnik Akademii meditsinskikh nauk SSSR","volume":" 6","pages":"55-7"},"PeriodicalIF":0.0000,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"[Immunobiological characteristics of Mycoplasma hyopneumoniae antigens and the diagnosis of infection caused by this organism].\",\"authors\":\"A Ia Pustovar\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>The paper presents the developed variants of enzyme immunoassay (EIA) using two M. hyopneumoniae (R-14 strain) antigens obtained by biomass freezing and thawing and two Tween-dissolved antigens by gel chromatography. To identify the cultures isolated, an indirect immunofluorescence reaction was proposed by employing agar blocks. The developed methods made it possible to identify 23 bovine-isolated cultures as M. hyopneumoniae antigens. The latter were detected by EIA in diagnostically significant titers in serum and colostrum samples from 2 swine-breeding farms. A negative result was obtained in the respiratory mycoplasmosis-free farms.</p>\",\"PeriodicalId\":76796,\"journal\":{\"name\":\"Vestnik Akademii meditsinskikh nauk SSSR\",\"volume\":\" 6\",\"pages\":\"55-7\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1991-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Vestnik Akademii meditsinskikh nauk SSSR\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Vestnik Akademii meditsinskikh nauk SSSR","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
[Immunobiological characteristics of Mycoplasma hyopneumoniae antigens and the diagnosis of infection caused by this organism].
The paper presents the developed variants of enzyme immunoassay (EIA) using two M. hyopneumoniae (R-14 strain) antigens obtained by biomass freezing and thawing and two Tween-dissolved antigens by gel chromatography. To identify the cultures isolated, an indirect immunofluorescence reaction was proposed by employing agar blocks. The developed methods made it possible to identify 23 bovine-isolated cultures as M. hyopneumoniae antigens. The latter were detected by EIA in diagnostically significant titers in serum and colostrum samples from 2 swine-breeding farms. A negative result was obtained in the respiratory mycoplasmosis-free farms.
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