{"title":"荧光法和比色法测定大鼠胸膜巨噬细胞产生过氧化氢的比较","authors":"Claudia Von Rein, Rolf Hirschelmann","doi":"10.1016/0160-5402(91)90054-9","DOIUrl":null,"url":null,"abstract":"<div><p>A fluorimetric and a colorimetric method for the determination of hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) production by isolated cells were compared. Despite a higher sensitivity of the fluorimetric assay, using homovanillic acid (HVA) as reagent, a significantly lower H<sub>2</sub>O<sub>2</sub> production by rat pleural macrophages was measured in comparison to the colorimetric phenol red method. A negative influence of HVA on H<sub>2</sub>O<sub>2</sub> production was detected in the colorimetric assay. These results suggest that the fluorimetric assay with HVA is unsuitable for determining H<sub>2</sub>O<sub>2</sub> formation by isolated cells.</p></div>","PeriodicalId":16819,"journal":{"name":"Journal of pharmacological methods","volume":"26 1","pages":"Pages 61-65"},"PeriodicalIF":0.0000,"publicationDate":"1991-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0160-5402(91)90054-9","citationCount":"4","resultStr":"{\"title\":\"Comparison of a fluorimetric and a colorimetric method for the determination of hydrogen peroxide production by rat pleural macrophages\",\"authors\":\"Claudia Von Rein, Rolf Hirschelmann\",\"doi\":\"10.1016/0160-5402(91)90054-9\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>A fluorimetric and a colorimetric method for the determination of hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) production by isolated cells were compared. Despite a higher sensitivity of the fluorimetric assay, using homovanillic acid (HVA) as reagent, a significantly lower H<sub>2</sub>O<sub>2</sub> production by rat pleural macrophages was measured in comparison to the colorimetric phenol red method. A negative influence of HVA on H<sub>2</sub>O<sub>2</sub> production was detected in the colorimetric assay. These results suggest that the fluorimetric assay with HVA is unsuitable for determining H<sub>2</sub>O<sub>2</sub> formation by isolated cells.</p></div>\",\"PeriodicalId\":16819,\"journal\":{\"name\":\"Journal of pharmacological methods\",\"volume\":\"26 1\",\"pages\":\"Pages 61-65\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1991-08-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/0160-5402(91)90054-9\",\"citationCount\":\"4\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of pharmacological methods\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/0160540291900549\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of pharmacological methods","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0160540291900549","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Comparison of a fluorimetric and a colorimetric method for the determination of hydrogen peroxide production by rat pleural macrophages
A fluorimetric and a colorimetric method for the determination of hydrogen peroxide (H2O2) production by isolated cells were compared. Despite a higher sensitivity of the fluorimetric assay, using homovanillic acid (HVA) as reagent, a significantly lower H2O2 production by rat pleural macrophages was measured in comparison to the colorimetric phenol red method. A negative influence of HVA on H2O2 production was detected in the colorimetric assay. These results suggest that the fluorimetric assay with HVA is unsuitable for determining H2O2 formation by isolated cells.
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