Immobilization of Fungal Peroxidase on Paramagnetic Nanoparticles for Synthetic Dye Decolorization

: Peroxidase was produced by Aspergillus flavus KIGC on 11 th day of fermentation when grown on banana peel as the sole carbon source. It was purified to 8.86 folds with percentage recovery of 49.72%. The purified peroxidase was immobilized on magnetic nanoparticle and character-ized using DLS, DSC, XRD, FTIR and SEM analyses. The results suggest adequate nanoparticle for-mulation and effective enzyme immobilization. Optimal temperatures for free and immobilized peroxidase were 75 and 70 °C, respectively, optimum pH of 5.0 and 9.0 were obtained for free enzyme and 6.5 and 9.0 for immobilized peroxidase, respectively. The Km of 0.075mM and catalytic efficiency of 90.66 Mol/S-1 were achieved by the immobilized peroxidase on guaiacol as substrate. The immobilized peroxidase exhibited enhanced synthetic dyes decolorization ability.