土芋贮藏腐烂的病原真菌及生物制剂的评价

P. Bora, K. Saikia, Shenaz Ahmed
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引用次数: 3

摘要

芋头(Colocasia esculenta)是印度广泛种植的主要热带块茎作物。芋块根和块茎收获后的损失已成为制约芋生产的主要因素之一,并可能对粮食安全产生不利影响。通过对黄瓜块茎贮藏腐病的研究发现,尖孢镰刀菌是造成黄瓜收获后损失的主要原因。致病性试验证实了其作为芋头贮藏腐病病原的作用。利用ITS1和ITS4扩增rDNA ITS区,进一步进行分子鉴定。BLST分析表明,该菌株与尖孢镰刀菌的同源性最高(95% ~ 99%)。结果表明,绿色木霉+荧光假单胞菌+苏云金芽孢杆菌联合处理对尖孢镰刀菌的抑菌率最高(94.05%),其次为绿色木霉单独处理(65.17%)。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Pathogenic fungi associated with storage rot of Colocasia esculenta and evaluation of bioformulations against the pathogen
Taro (Colocasia esculenta) is a major tropical tuber crop widely cultivated in India. Post-harvest loss of C. esculenta root and tubers had emerged as one of the major constraints in the production of taro and might have detrimental effects on food security. Our study on storage rot of C. esculenta tubers revealed that Fusarium oxysporum was mostly responsible for the post-harvest loss of the crop. Pathogenicity test confirmed its role as the causal agent of storage rot in taro. The pathogen was further confirmed based on molecular identification by amplifying ITS region of the rDNA using ITS1 and ITS4. Based on BLST analysis it showed maximum homology (95-99%) with F. oxysporum. Efficacy of a few microbial antagonists were tested against the pathogen in vitro which revealed that the treatment with combination of Trichoderma viride + Pseudomonas fluorescens + Bacillus thuringiensis showed highest inhibition of F. oxysporum (94.05%) followed by treatment of T. viride alone (65.17%).
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