犬颈总动脉分级狭窄周围的内皮细胞形态。

Blood vessels Pub Date : 1991-01-01 DOI:10.1159/000158886
K J Hutchison
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引用次数: 13

摘要

麻醉下对犬颈总动脉进行分级狭窄(缩小40-64%直径),27天后经皮脉冲多普勒测速仪检测狭窄后的速度场。原位固定后,通过扫描电镜对内皮细胞轮廓进行数字化,测量内皮细胞的面积、形状和方向。狭窄喉部附近(+/- 1直径内)内皮细胞面积减小。与狭窄血流动力学无明显关系。细胞面积向下游增加5个直径;在这一地区,由于急流破裂,出现了湍流。细胞在狭窄喉部最大圆形,并逐渐向上游延伸5个直径。在狭窄处和最大湍流位置之间存在一个流动分离和低速再循环区域,因此下游胞块的圆切与流动分离有关。将实验分为四个狭窄等级,各组之间的形态模式变化不大。这可能与随品位增加而产生的流动限制有关。内皮细胞通过狭窄入口的伸长仅在四个实验中明显。在16个实验中,只有4个实验发现狭窄后扩张大于外径增加12%。一般来说,细胞伸长与剪切应力的关系得到了支持,但这种关系并不能完全解释狭窄处内皮细胞的形状。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Endothelial cell morphology around graded stenoses of the dog common carotid artery.

Graded stenoses (40-64% diameter reduction) were applied to dog common carotid arteries under anesthesia, and 27 days later the poststenotic velocity field was examined using transcutaneous pulsed Doppler velocimetry. Following in situ fixation, endothelial cell area, shape and orientation was measured by digitizing endothelial cell outlines in scanning electron micrographs. Endothelial cell area in the vicinity of the stenosis throat (within +/- 1 diameter) was reduced. No relation to stenosis hemodynamics was apparent. Cell area was increased 5 diameters downstream; in this region, there was turbulence due to jet break up. Cells were maximally rounded at the stenosis throat and gradually returned to upstream elongation by 5 diameters downstream. There was a region of flow separation and low velocity recirculation between the stenosis and the site of maximum turbulence, thus, downstream cell rounding was associated with flow separation. Subdivision of the experiments into four stenosis grades revealed little variation of the pattern of morphology between groups. It is possible that this is related to flow limitation with increasing grade. Elongation of endothelial cells through the entrance of the stenosis was apparent in only four experiments. Poststenotic dilatation of greater than 12% external diameter increase was noted in only four out of the sixteen experiments. In general, the relation of cell elongation to shear stress is supported, but this relationship does not fully explain endothelial cell shape at a stenosis.

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