雌激素受体在非洲爪蟾卵黄原基因表达中的作用。

M. Hayward, Martin L. Brock, David J Shapiro
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引用次数: 67

摘要

雌二醇17 β[雌二醇-1,3,5(10)-三烯-3,17- β -二醇]可诱导雄性非洲爪蟾肝脏大量合成蛋黄前体磷脂糖蛋白、卵黄原蛋白及其同源mrna。先前已被诱导合成卵黄原蛋白mRNA,但在雌激素的再刺激下,卵黄原蛋白mRNA的合成处于失活状态的雄性黄颡鱼会比初次雌激素刺激后更快地积累卵黄原蛋白mRNA,更有效地转录卵黄原蛋白基因。在这种生物体中,雌激素受体系统介导雌激素的作用,表现出几个不寻常的特性。未受刺激的肝细胞的细胞质含有高水平的中等亲和力的雌激素特异性结合蛋白和很少的雌激素受体。雌激素结合蛋白的特性与保护雌二醇17 β抗代谢的作用是一致的,因为细胞质中雌二醇17 β的一部分不受快速代谢的影响。此外,在所有对类固醇激素有反应的爪蟾组织中发现了类似的结合活性。核雌激素受体的诱导与卵黄原蛋白mRNA积累的开始一致。然而,雌激素受体水平的升高并不是雌激素再刺激后卵黄蛋白原基因转录率升高的原因。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The role of estrogen receptor in Xenopus laevis vitellogenin gene expression.
Administration of estradiol 17 beta [estra-1,3,5(10)-triene-3,17-beta-diol] to male Xenopus laevis induces the massive synthesis by the liver of the egg yolk precursor phospholipoglycoprotein, vitellogenin, and its cognate mRNAs. Restimulation of male X. laevis that have been previously induced to synthesize vitellogenin mRNA but are inactive in vitellogenin mRNA synthesis at the time of restimulation with estrogen results in more rapid accumulation of vitellogenin mRNA and more efficient transcription of the vitellogenin genes than occurs following primary estrogen stimulation. The estrogen receptor system that mediates estrogen action in this organism exhibits several unusual properties. The cytoplasm of unstimulated liver cells contains high levels of a middle-affinity estrogen-specific binding protein and little if any estrogen receptor. The properties of the estrogen binding protein are consistent with a role in protecting estradiol 17 beta against metabolism, as a fraction of cytoplasmic estradiol 17 beta is not subject to rapid metabolism. In addition, similar binding activities are found in all Xenopus tissues surveyed that respond to steroid hormones. The induction of nuclear estrogen receptor is coincident with the onset of vitellogenin mRNA accumulation. However, an increased level of estrogen receptor is not responsible for the elevated rate of vitellogenin gene transcription observed following restimulation with estrogen.
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