使用聚合纳米颗粒的抗逆转录病毒疫苗

M. Akashi, T. Akagi, T. Kaneko, M. Baba, M. Hayami
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引用次数: 0

摘要

我们之前报道过,芋豆蛋白a固定化聚苯乙烯纳米球(Con A-NS)可以有效捕获HIV-1颗粒,并且用灭活的HIV-1捕获纳米球(HIV-NS)经鼻免疫可诱导小鼠阴道抗HIV-1 IgA抗体反应。在这项研究中,为了评估免疫的保护作用,每3只猕猴鼻内注射Con a - ns或灭活的猴/人免疫缺陷病毒KU-2捕获纳米球(SHIV- ns),然后阴道内注射一种致病性病毒SHIV KU-2。经过6次免疫后,所有shiv - ns免疫的猕猴阴道均检测到抗hiv -1 gp120 IgA和IgG抗体。经阴道内攻击后,Con A-NS和shiv - ns免疫组各有一只猕猴被感染。shiv - ns免疫的感染猕猴血浆病毒RNA载量明显低于未免疫的对照猕猴,达到可检测水平以下。因此,SHIV- ns免疫的猕猴对SHIV KU-2阴道攻击表现出部分保护作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Anti-Retroviral Vaccine Using Polymeric Nanoparticles
We have previously reported that concanavalin A-immobilized polystyrene nanospheres (Con A-NS) could efficiently capture HIV-1 particles and that intranasal immunization with inactivated HIV-1- capturing nanospheres (HIV-NS) induced vaginal anti-HIV-1 IgA antibody response in mice. In this study, to evaluate the protective effect of immunization, each three macaques was intranasally immunized with Con A-NS or inactivated simian/human immunodeficiency virus KU-2- capturing nanospheres (SHIV-NS) and then intravaginally challenged with a pathogenic virus, SHIV KU-2. After a series of six immunizations, vaginal anti-HIV-1 gp120 IgA and IgG antibodies were detected in all SHIV-NS-immunized macaques. After intravaginal challenge, one of the three macaques in each of the Con A-NS- and SHIV-NS-immunized groups was infected. Plasma viral RNA load of infected macaque in SHIV-NS-immunized macaques was substantially less than that in unimmunized control macaque and reached below the detectable level. Thus, SHIV-NS-immunized macaques exhibited partial protection to vaginal challenges with SHIV KU-2.
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