{"title":"西加里曼丹当地的gella sonnei乙醇乙醇提取物的抗菌活性","authors":"Laili Fitri Yeni","doi":"10.25134/quagga.v14i1.5081","DOIUrl":null,"url":null,"abstract":"Shigella sonnei merupakan salah satu bakteri patogen penyebab disentri basiler dengan tingkat prevalensi tinggi di negara berkembang. Bakteri ini dilaporkan resisten terhadap beberapa antibiotik yang direkomendasikan WHO, sehingga diperlukan upaya pencarian senyawa antibakteri baru. Penelitian ini bertujuan untuk mendeskripsikan kandungan fitokimia dan potensi ekstrak etanol umbi, batang dan daun ginseng jawa (Talinum paniculatum) dalam menghambat pertumbuhan S. sonnei secara in vitro. Uji fitokimia ekstrak etanol menggunakan metode Kromatografi Lapis Tipis (KLT). Potensi antibakteri dilakukan dengan menerapkan metode difusi cakram Kirby Baeur teknik swab dengan perlakuan seri konsentrasi ekstrak 50%, 75% dan 100%, kontrol positif (tetrasiklin 20%), dan kontrol negatif (DMSO 10%) dengan 5 pengulangan. Dari hasil uji fitokimia diketahui bahwa metabolit sekunder dari golongan steroid ditemukan pada umbi, batang maupun daun ginseng jawa. Flavonoid hanya ditemukan pada umbi dan daun. Ekstrak etanol batang dan daun menunjukkan hasil positif untuk tanin dan fenolik. Alkaloid, saponin dan terpenoid tidak ditemukan pada ketiga ekstrak tersebut. Ekstrak etanol daun memiliki potensi penghambatan terhadap S. sonnei lebih besar dibandingkan umbi dan batang, terutama pada konsentrasi 100%. Meskipun demikian zona hambat yang terbentuk masih belum cukup efektif jika dibandingkan dengan zona hambat yang dibentuk oleh kontrol positif yaitu tetrasiklin. Shigella sonnei a pathogenic bacterium that causes bacillary dysentery – a disease with high prevalence in developing countries. This bacterium has been reported resistant to several antibiotics. Development of new antibacterial compounds is regarded necessary. This study was conducted to describe the phytochemical content and potentials of ethanol extract from the tubers, stems and leaves of fameflowers (Talinum paniculatum) in inhibiting the growth of S. sonnei in vitro. Phytochemical test was performed to the ethanol extract using Thin Layer Chromatography (TLC). Antibacterial potential was assessed using Kirby Baeur disc diffusion swab on different treatment concentrations of 50%, 75% and 100% with positive control (20% tetracycline), and negative control (DMSO 10%) in 5 replications. The phytochemical tests showed that secondary metabolites from the steroid group were found in tubers, stems and leaves of the famefolowers. However, flavonoids were only found in tubers and leaves. The ethanol extracts of stems and leaves also contained tannins and phenolics. Furthermore, neither Alkaloids, saponins or terpenoids were found in the three extracts. In addition, ethanol extract from the leaves showed stronger inhibitory potential against S. sonnei extracts from tubers and stems, particularly at 100% concentration. However, the inhibition zone formed in the extracts was still less effective compared to the inhibition zone formed by tetracycline as positive control.","PeriodicalId":305827,"journal":{"name":"Quagga: Jurnal Pendidikan dan Biologi","volume":"5 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2022-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"Aktivitas Antibakteri Ekstrak Etanol Talinum paniculatum Lokal Kalimantan Barat terhadap Shigella sonnei\",\"authors\":\"Laili Fitri Yeni\",\"doi\":\"10.25134/quagga.v14i1.5081\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Shigella sonnei merupakan salah satu bakteri patogen penyebab disentri basiler dengan tingkat prevalensi tinggi di negara berkembang. Bakteri ini dilaporkan resisten terhadap beberapa antibiotik yang direkomendasikan WHO, sehingga diperlukan upaya pencarian senyawa antibakteri baru. Penelitian ini bertujuan untuk mendeskripsikan kandungan fitokimia dan potensi ekstrak etanol umbi, batang dan daun ginseng jawa (Talinum paniculatum) dalam menghambat pertumbuhan S. sonnei secara in vitro. Uji fitokimia ekstrak etanol menggunakan metode Kromatografi Lapis Tipis (KLT). Potensi antibakteri dilakukan dengan menerapkan metode difusi cakram Kirby Baeur teknik swab dengan perlakuan seri konsentrasi ekstrak 50%, 75% dan 100%, kontrol positif (tetrasiklin 20%), dan kontrol negatif (DMSO 10%) dengan 5 pengulangan. Dari hasil uji fitokimia diketahui bahwa metabolit sekunder dari golongan steroid ditemukan pada umbi, batang maupun daun ginseng jawa. Flavonoid hanya ditemukan pada umbi dan daun. Ekstrak etanol batang dan daun menunjukkan hasil positif untuk tanin dan fenolik. Alkaloid, saponin dan terpenoid tidak ditemukan pada ketiga ekstrak tersebut. Ekstrak etanol daun memiliki potensi penghambatan terhadap S. sonnei lebih besar dibandingkan umbi dan batang, terutama pada konsentrasi 100%. Meskipun demikian zona hambat yang terbentuk masih belum cukup efektif jika dibandingkan dengan zona hambat yang dibentuk oleh kontrol positif yaitu tetrasiklin. Shigella sonnei a pathogenic bacterium that causes bacillary dysentery – a disease with high prevalence in developing countries. This bacterium has been reported resistant to several antibiotics. Development of new antibacterial compounds is regarded necessary. This study was conducted to describe the phytochemical content and potentials of ethanol extract from the tubers, stems and leaves of fameflowers (Talinum paniculatum) in inhibiting the growth of S. sonnei in vitro. Phytochemical test was performed to the ethanol extract using Thin Layer Chromatography (TLC). Antibacterial potential was assessed using Kirby Baeur disc diffusion swab on different treatment concentrations of 50%, 75% and 100% with positive control (20% tetracycline), and negative control (DMSO 10%) in 5 replications. The phytochemical tests showed that secondary metabolites from the steroid group were found in tubers, stems and leaves of the famefolowers. However, flavonoids were only found in tubers and leaves. The ethanol extracts of stems and leaves also contained tannins and phenolics. Furthermore, neither Alkaloids, saponins or terpenoids were found in the three extracts. In addition, ethanol extract from the leaves showed stronger inhibitory potential against S. sonnei extracts from tubers and stems, particularly at 100% concentration. However, the inhibition zone formed in the extracts was still less effective compared to the inhibition zone formed by tetracycline as positive control.\",\"PeriodicalId\":305827,\"journal\":{\"name\":\"Quagga: Jurnal Pendidikan dan Biologi\",\"volume\":\"5 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2022-01-10\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Quagga: Jurnal Pendidikan dan Biologi\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.25134/quagga.v14i1.5081\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Quagga: Jurnal Pendidikan dan Biologi","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.25134/quagga.v14i1.5081","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 1
摘要
Shigella sonnei是病原体导致痢疾basiler之一和高水平的患病率在发展中国家。这是世卫组织推荐的报告对一些抗生素产生抗药性细菌,所以需要努力寻找新的抗菌化合物。本研究的目的是描述乙醇提取物块茎植物化学成分和潜力,爪哇人参茎和叶(Talinum paniculatum)成长的阻碍中S sonnei体外地。乙醇萃取植物化学试验采用薄层色谱法。抗菌潜力是通过使用Kirby berur的磁盘扩散技术swab处理一系列提取50%、75%和100%的浓度、正控制(20%的四环素)和负控制(DMSO 10%的重复)来实现的。从阶级的植物化学测试知悉次生代谢类固醇在块茎,茎和叶发现爪哇人参。块茎类黄酮,只在发现和树叶。茎和叶乙醇提取物对单宁酸和酚表示积极的结果。在这三种提取物中都没有发现生物碱、参本和松节油。乙醇提取物比块茎和茎更有可能抑制水分,尤其是在100%的浓度下。然而拖住还不够有效形成的区域相比,拖住控制由积极的区域即四环素。Shigella sonnei a pathogenic bacterium那敢死队bacillary dysentery——疾病和发展中国家高prevalence in developing a。这个bacterium已被reported resistant to好几个注射抗生素。Development of new antibacterial compounds regarded是必要的。这个to study was conducted描述乙醇extract phytochemical内容和potentials》从《fameflowers tubers, stems和树叶(Talinum paniculatum)在体外sonnei增长》inhibiting S。Phytochemical测试是performed乙醇extract用瘦》层层Chromatography (TLC)。用科比Baeur Antibacterial潜在的was assessed光盘上不同的治疗concentrations of the diffusion拭子50%、75%和100%积极控制四环素(20%),和在5 replications负10%的控制(DMSO)。《phytochemical测试那里那这metabolites从《tubers类固醇集团发现,stems》和《famefolowers树叶。但是,flavonoids是只发现在tubers和树叶。stems乙醇摘录》和树叶也有趣tannins和phenolics。Furthermore,也生物碱,saponins或terpenoids》发现三个摘录。在加法,从树叶那里,乙醇extract inhibitory强潜在的反S . sonnei摘录tubers和stems,特别的100%双臀。但是,境抑制区formed摘录少仍然是有效compared to the抑制formed区由美国四环素积极控制。
Aktivitas Antibakteri Ekstrak Etanol Talinum paniculatum Lokal Kalimantan Barat terhadap Shigella sonnei
Shigella sonnei merupakan salah satu bakteri patogen penyebab disentri basiler dengan tingkat prevalensi tinggi di negara berkembang. Bakteri ini dilaporkan resisten terhadap beberapa antibiotik yang direkomendasikan WHO, sehingga diperlukan upaya pencarian senyawa antibakteri baru. Penelitian ini bertujuan untuk mendeskripsikan kandungan fitokimia dan potensi ekstrak etanol umbi, batang dan daun ginseng jawa (Talinum paniculatum) dalam menghambat pertumbuhan S. sonnei secara in vitro. Uji fitokimia ekstrak etanol menggunakan metode Kromatografi Lapis Tipis (KLT). Potensi antibakteri dilakukan dengan menerapkan metode difusi cakram Kirby Baeur teknik swab dengan perlakuan seri konsentrasi ekstrak 50%, 75% dan 100%, kontrol positif (tetrasiklin 20%), dan kontrol negatif (DMSO 10%) dengan 5 pengulangan. Dari hasil uji fitokimia diketahui bahwa metabolit sekunder dari golongan steroid ditemukan pada umbi, batang maupun daun ginseng jawa. Flavonoid hanya ditemukan pada umbi dan daun. Ekstrak etanol batang dan daun menunjukkan hasil positif untuk tanin dan fenolik. Alkaloid, saponin dan terpenoid tidak ditemukan pada ketiga ekstrak tersebut. Ekstrak etanol daun memiliki potensi penghambatan terhadap S. sonnei lebih besar dibandingkan umbi dan batang, terutama pada konsentrasi 100%. Meskipun demikian zona hambat yang terbentuk masih belum cukup efektif jika dibandingkan dengan zona hambat yang dibentuk oleh kontrol positif yaitu tetrasiklin. Shigella sonnei a pathogenic bacterium that causes bacillary dysentery – a disease with high prevalence in developing countries. This bacterium has been reported resistant to several antibiotics. Development of new antibacterial compounds is regarded necessary. This study was conducted to describe the phytochemical content and potentials of ethanol extract from the tubers, stems and leaves of fameflowers (Talinum paniculatum) in inhibiting the growth of S. sonnei in vitro. Phytochemical test was performed to the ethanol extract using Thin Layer Chromatography (TLC). Antibacterial potential was assessed using Kirby Baeur disc diffusion swab on different treatment concentrations of 50%, 75% and 100% with positive control (20% tetracycline), and negative control (DMSO 10%) in 5 replications. The phytochemical tests showed that secondary metabolites from the steroid group were found in tubers, stems and leaves of the famefolowers. However, flavonoids were only found in tubers and leaves. The ethanol extracts of stems and leaves also contained tannins and phenolics. Furthermore, neither Alkaloids, saponins or terpenoids were found in the three extracts. In addition, ethanol extract from the leaves showed stronger inhibitory potential against S. sonnei extracts from tubers and stems, particularly at 100% concentration. However, the inhibition zone formed in the extracts was still less effective compared to the inhibition zone formed by tetracycline as positive control.